Zhejiang Hisun Pharmaceutical Co., Ltd.

Linghui Zheng

Hua Bai

Na Li

Jun Huang

Meihong Li

Haibin Wang

Huijun Ren

Zhen Yu

Feng Chen

Jidong Wang

Hao Xu

Min Zhou

Lingping Wang

Fei Tang

Peijun Zhan

Jianhong Hu

Hongmei Fan

Xuexiao Ying

Daochao Li

Jiatan Lin

Aiwen Deng

Anliang Chen

Yingying He

Shuang Han

Xing Jiang

The present invention relates to a DoxA protein mutant having an amino acid sequence set forth in SEQ ID NO: 16, and coding gene thereof. The protein mutant or the coding gene thereof can be used for producing epirubicin. The present invention further relates to acapable of efficiently expressing epirubicin, which is constructed by replacing the dnmV gene of a startingin situ with the avrE gene and mutating the doxA gene of the startinginto a gene encoding the protein set forth in SEQ ID NO: 16. The fermentation broth of thishas an epirubicin potency of up to 102.0 &#x3bc;g/ml.

DoxA protein mutant, and coding gene and applications thereof

The invention provides a construction method of spinosad heterologous expression strain, a spinosad heterologous expression strain obtained by the method and use thereof in preparing spinosad. The method utilizes a plurality of homologous recombination to replace the erythromycin synthetic gene cluster ofwith the spinosad synthetic gene cluster and the rhamnose synthetic gene cluster, such that theproduces spinosad.

Spinosad heterologous expression strain and construction method thereof and use

The present invention relates to a recombinant microorganism expressing avermectin or analogs thereof and construction method thereof, and also relates to a method of producing avermectin or analogs thereof using the recombinant microorganism, and avermectin or analogs thereof obtained using the method. In addition, the present invention further relates to uses of the avermectin or analogs thereof as insecticides. Using the recombinant microorganism of the present invention to produce avermectin or analogs thereof has numerous advantages, for example comprising at least one of the following: good stability, high yield, simple process, environmentally friendly, and greatly saving of production costs.

Recombinant microorganism expressing avermectin analogue and use thereof

A fidaxomicin purification method, comprising: fermentingsp. HS-16-20 to generate fermented liquid; conducting solid/liquid separation on the fermented liquid, soaking mycelium in an organic solvent, and filtering to obtain a solution containing fidaxomicin; treating the solution with nanofiltration concentrate, and separating to obtain fidaxomicin crude product; conducting preparative column chromatography on the fidaxomicin crude product, eluting with an acid aqueous solution containing an organic solvent, and separating to obtain the refined fidaxomicin product.

Fidaxomicin purification method

A bacterial strain ofsp. and and the use thereof. The bacterial strain is namedsp. HS-16-20, whose preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 7294. The bacterial strain can be used to generate Fidaxomicin or analogs thereof or compositions containing Fidaxomicin, such as by aerobically fermenting the strain in nutrient medium containing assimilable carbon and/or nitrogen sources. Fidaxomicin has an inhibitory effect on various gram positive bacteria pathogens, and in particular, on

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Zhejiang, China

Linghui Zheng