Harvard Apparatus Regenerative Technology, Inc.

Biostage, Inc.

Case Western Reserve University

Linghui Meng

Sherif Soliman

Shunfu Hu

William Fodor

Saverio La Francesca

David L Green

Gary E Wnek

Joseph Consiglio

Ron Sostek

Matthew Marsh

Aspects of the disclosure relate methods and a synthetic cell delivery device for treating trauma present relative to the inner surface of a hollow organ such as an esophagus.

Method and device for in vivo tissue regeneration on the interior surface of hollow organs

Aspects of the disclosure relate methods and synthetic scaffolds for regenerating hollow tubular organs present in the respiratory system such as bronchus tissue.

Method and device for in vivo bronchus regeneration

Aspects of the disclosure relate methods and synthetic scaffolds for regenerating gastrointestinal tissue (e.g., esophageal tissue).

Systems and methods for producing gastrointestinal tissues at an anastomosis or other physiological location

Aspects of the disclosure relate to synthetic tissue or organ scaffolds and methods and compositions for promoting or maintaining their structural integrity. Aspects of the disclosure are useful to prevent scaffold damage (e.g., delamination) during or after implantation into a host. Aspects of the disclosure are useful to stabilize tissue or organ scaffolds that include electrospun fibers.

Methods and compositions for promoting the structural integrity of scaffolds for tissue engineering

Systems and methods for producing gastrointestinal tissues

Aspects of the disclosure relate methods and synthetic scaffolds for regenerating hallow organs present in the respiratory system such as bronchus tissue.

A method of forming a cross-linked protein structures includes preparing a solution of protein dissolved in a benign solvent and forming an intermediate protein structure from the solution. The intermediate protein structure can be cross-linked by providing for a specific ratio of chemical cross-linking agents to form the cross-linked protein structure. The solution can be prepared by adding a cross-linker of N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) at a ratio of two-to-one of NHS to EDC to alcohol. PBS buffer (20&#xd7;) can be added to the solution until the volume ratio of PBS buffer (20&#xd7;) to alcohol is about one-to-one. About 16 percent by weight of protein can be dissolved in the solution. The solution can be electrospun to form an intermediate protein structure. After a period of time, the protein structure can be cross-linked to form the cross-linked protein structure.

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Holliston, MA, United States of America

Linghui Meng