Zhejiang University

Linfu Zhou

Zhi Kuan Chen

Yanshan Huang

Min Zheng

Shuangshuang Li

Guohua Lou

Jiajia Shao

Zhi Chen

The present application belongs to the field of genetic engineering and medical diagnosis, and particularly relates to serum miRNA markers for diagnosing liver cancer and predicting liver cancer metastasis and a detection kit thereof. The present application discloses the research and development and use of a kit containing three blood miRNAs markers related to human liver cancer. The specific markers are serum-derived miR-122-5p, miR-144-3p and miR-451a. According to the present application, the differential miRNAs are used as the core components of the detection kit, and are combined with corresponding internal references to form the detection kit; and through practical detection application in clinical samples, the reaction conditions and reagent ratios of the kit are optimized, the detection parameters are adjusted, and the standardized process of clinical detection and analysis is preliminarily established, and the kit is used for early diagnosis and metastasis risk assessment of liver cancer.

Serum miRNA markers for diagnosing liver cancer and predicting liver cancer metastasis and detection kit thereof

This present invention relates to compositions comprising biological active proteins fused with extended recombinant gelatin-like protein (GLK), nucleic acids encoding the compositions and vectors containing the same, approaches involved in the preparation of the compositions and their pharmaceutical application in the treatment or prevention of diseases.

Nonnatural collagen-like protein and use thereof

Growing community of inventors

Hangzhou, China

Linfu Zhou