Bolt Threads, Inc.

Tufts University

University of Pavia

Lindsay Wray

Joshua Kittleson

David N Breslauer

Daniel M Widmaier

Brendan Turner

David L Kaplan

Maxime Boulet-Audet

Hua Li

Steven Joseph Tom

Nicole Elizabeth Subler

Zoya Nasir Zulfiqar

Amir Ahmad Bakhtiary Davijani

Jessica Wang

Paul Andre Guerette

Loren A Perelman

Lorenzo Tozzi

Christian Andrea Di Buduo

Alessandra Balduini

Jelena Rnjak-Kovacina

Josh Kittleson

Jamie Mcausland Bainbridge

Provided herein are scalable methods of processing wet-spun fiber comprising recombinant spider silk polypeptides to generate a three-dimensional crystalline lattice of beta-sheet structures in the fiber.

Methods of generating highly-crystalline recombinant spider silk protein fibers

The present invention provides, among other things, methods for producing platelets including the steps of providing a silk membrane about 2 &#x3bc;m and 100 &#x3bc;m thick, inclusive, contacting the silk membrane with a porogen to form a porous silk membrane comprising at least one silk wall defining a lumen, associating the porous silk membrane with stromal derived factor-1? and at least one functionalizing agent, forming a three dimensional silk matrix comprising interconnected pores wherein the pores have a diameter of between about 5 and 500 &#x3bc;m, inclusive, wherein the silk matrix is formed around at least a portion of the porous silk membrane, introducing a plurality of megakaryocytes to the silk matrix such that the megakaryocytes are located at least partially within the porous silk matrix, and stimulating the plurality of megakaryocytes to produce platelets. Also provided are various new compositions and methods of making those compositions.

Microphysiologic methods and compositions

The present disclosure provides methods and compositions for directed to synthetic block copolymer proteins, expression constructs for their secretion, recombinant microorganisms for their production, and synthetic fibers (including advantageously, microfibers) comprising these proteins that recapitulate many properties of natural silk. The recombinant microorganisms can be used for the commercial production of silk-like fibers.

Methods and compositions for synthesizing improved silk fibers

The present disclosure provides improved long uniform recombinant protein fibers with desirable physical traits. The present disclosure also provides compositions derived from the long uniform recombinant protein fibers.

Long uniform recombinant protein fibers

The inventions provided herein relate to silk-based scaffolds and methods of producing the same, which can be used for a range of tissue engineering applications. The fabrication methods described herein provide a versatile platform to incorporate hollow conduits (e.g., for nutrient/oxygen delivery) through three-dimensional silk-based scaffolds that have tunable bulk properties (e.g., but not limited to, porosity, mechanical, degradation rate) and allow endothelialization and/or cell compartmentalization, for engineering a variety of complex tissue equivalents.

Silk-based scaffold platform for engineering tissue constructs

Growing community of inventors

Benicia, CA, United States of America

Lindsay Wray