Qiagen Gmbh

The Council of the Queensland Institute of Medical Research

Molecular Staging Inc.

Australian Red Cross Society

Lindsay Colin Wolter

James Langham Dale

Gregory John Hafner

Philip Morrison Giffard

Mark Richard Stafford

Ilin Chen Hai-Ni Yang

Joanne Voisey

Catherine Hyland

Allan Saul

The present invention relates generally to a method of amplifying closed circular nucleic acid probes and, more particularly, to a method of amplifying closed circular nucleic acid probes by rolling circle amplification. The method of the present invention is useful in a range of applications involving the detection of nucleic acid sequences such as, but not limited to, the identification of genetic disorders, genetic variants or the presence of microbiological or viral agents.

Methods of forming circular nucleic acid probes and uses thereof

Method of amplification

A method of detecting a gene and quantifying its copy number using a PCR based assay is described. A set (i.e. three primers) of PCR primers is used in a single reaction wherein the first primer is specific for a first gene and the second primer is specific for a second gene which second gene is closely related to the first gene, while the third primer is common to both the first and second genes. From the results obtained the presence or absence of the second gene can be determined, as well as, its copy number.

Quantitative PCR-based method of gene detection

Growing community of inventors

Holland Park, Australia

Lindsay Colin Wolter