The General Hospital Corporation

Lindong Weng

Mehmet Toner

Shannon Stott

Shannon Tessier

Mustafa Korkut Uygun

Stephanie E J Cronin

Reinier Johan De Vries

Gloria Y Lee

Casie A Pendexter

Microfluidic systems are configured to process liquid samples that include cumulus-oocyte complexes (COCs), such as raw follicular fluid, in an automated and continuous manner to produce oocytes separate or 'denuded' from surrounding cumulus cells. The systems include a substrate and a channel that can have two or more sub-channels. Each channel includes an inlet, an outlet, and one or more stages arranged in series. Each of the stages includes one or more expansion units and one or more constriction units. At least one stage includes constriction units having jagged internal surfaces, e.g., teeth, that help remove the cumulus cells from the COCs.

Microfluidic systems and methods to denude mammalian oocytes

This disclosure is related to methods of preserving biological samples, organs, and organisms. This disclosure is based, at least in part, on the discovery that in the presence of heterogenous extracellular ice, endothelial cells have improved attachment and viability after preservation, and particularly by uniformly nucleating ice across the microvasculature using ice nucleating agents, the chance of intracelluar ice formation can be significantly reduced.

High subzero cryopreservation

This disclosure relates to ice nucleation formulations for cryopreservation and stabilization of biologics, and methods of use thereof.

Ice nucleation formulations for cryopreservation and stabilization of biologics

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Arlington, MA, United States of America

Lindong Weng