One Lambda, Inc.

University of California

Chiron Corporation

International Genetic Engineering

Zoma Corporation

Lindley Blair

Jar-How Lee

David J Julius

Katsuyuki Saito

Raju K Koduri

Arnold H Horwitz

Anthony J Brake

Ira Herskowitz

Joachim L Weickmann

Jeremy W Thorner

Anthony Brake

Jeremy Thorner

George Sprague

The invention provides methods for detecting target nucleic acid sequences with diagnostic probes including first and second probe regions that are substantially complementary to first and second target regions respectively on a target nucleic acid strand wherein the first probe region is located 5' to the second probe region. The first probe region is substantially complementary to the first target region, on the target nucleic acid strand, which also includes a second target region, wherein when the first target region is contiguous with the second target region on the target nucleic acid strand, then the first and second probe regions on the diagnostic probe are separated by a spacer region of nucleic acid.

Diagnostic probe detection system

The invention provides methods for detecting target nucleic acid sequences with diagnostic probes including first and second probe regions that are substantially complementary to first and second target regions respectively on a target nucleic acid strand wherein the first probe region is located 5' to the second probe region. The first probe region is substantially complementary to the first target region, on the target nucleic acid strand, which also includes a second target region, wherein when said first target region is contiguous with the second target region on the target nucleic acid strand, then the first and second probe regions on the diagnostic probe are separated by a spacer region of nucleic acid.

The invention provides methods for detecting target nucleic acid sequences with diagnostic primers including priming regions and probe regions which are complementary to target and reference regions respectively on a sample nucleic acid strand wherein the probe region is located 5&prime; to the priming region which is complementary to a reference nucleic acid sequence which is 3&prime; to the target nucleic acid sequence on the sample nucleic acid strand wherein when said reference nucleic acid sequence is contiguous with said target nucleic acid sequence on the sample nucleic acid strand then the priming region and probe region on the diagnostic probe are separated by a spacer region of nucleic acid.

Method for amplification of DNA

Genes are disclosed which are capable of directing the synthesis in a selected host microorganism of thaumatin I polypeptides having improved taste characteristics characterized by having the amino acid sequence of natural thaumatin I including a lysine amino acid residue substituted for asparagine in the 46th position from the amino terminal end, an aspartate amino acid residue substituted for asparagine in the 113th position from the amino terminal end and an aspartate amino acid residue substituted for lysine at either the 97th or 137th residue from the amino terminal end.

DNA encoding (Lys.sup.46, Asp.sup.97, Asp.sup.113) and (Lys.sup.46, Asp.sup

A shortened PGK promoter ranging from 165 to 404 base pairs of the Saccharomyces PGK promoter is taught. The promoter is operably linked to heterologous genes and improves the expression thereof relative to the full length PGK promoter.

Shortened phosphoglycerate kinase promoter

Methods and compositions are provided for endopeptidase production, enhanced efficiencies of processing in vivo and in vitro to provide for process polypeptides, and purified enzyme for in vitro processing of polypeptides. The endopeptidase is specific for dibasic amino acid sites, cleaving at the C-side of the dipeptide. The S. cerevisiae strain pYBCA-5 (truncated KEX2) was deposited at the A.T.C.C. on June 21, 1984 and given Accession No. 20717.

Yeast endopeptidase for basic amino-acid site cleavage, preparation and

A method is provided for expressing the proteases dipeptidyl aminopeptidases A and B which are useful for processing precursor proteins. Genes controlling these proteases may be insdrted into appropriate vectors and transformed into cultures. The proteases may either be utilized to process precursor proteins in vivo or may be extracted from cultures and used to process precursor proteins in vitro.

Growing community of inventors

Los Angeles, CA, United States of America

Lindley Blair