Life Technologies Corporation

Linda Wong

Chunmei Liu

Shoulian Dong

Caifu Chen

Yalei Wu

Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise tailing both the 5' and 3&#x2032; ends of mature small RNA by ligating a 5&#x2032; ligation adaptor to the 5&#x2032; end and polyadenylating the 3&#x2032; end. Other embodiments comprise reverse transcribing the adaptor ligated, polyadenylated mature small RNA with a universal reverse transcription primer and amplifying the cDNA with universal primers.

Methods, compositions and kits for small RNA capture, detection and quantification

Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5' and 3&#x2032; ligation adaptors to the 5&#x2032; and 3&#x2032; ends of the mature small RNAs, respectively, in the presence of 5&#x2032; and 3&#x2032; semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3&#x2032; end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA ligation product.

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San Bruno, CA, United States of America

Linda Wong