Her Majesty the Queen in Right of Canada, As Represented by the Minister of Agriculture and Agri-food

Agri-food Canada

Her Majesty the Queen in Right of Canada, As Represented by the Minister

Her Majesty in Right of Canada, As Represented by the Minister of

Her Majesty the Queen in Right of Canada As Represented by the Minister of Agriculture

Linda J Harris

John A Simmonds

Leslie Cass

Sharon Allard

Brian Miki

Teresa Martin-Heller

Keqiang Wu

Stephen Gleddie

Kamal Malik

Dan Brown

Steve Gleddie

Ming Hu

This invention is directed to a regulatory region obtained from a wheat aleurone gene LtpW1. This regulatory region, truncated derivatives, mutations, or deletions of this regulatory region, can be used to express heterologous genes of interest within aleurone cells of a plant. Furthermore, this invention is directed to a truncated LtpW1 regulatory region that exhibits constitutive activity with both monocot and dicot plants. This invention is also directed to vectors comprising these regulatory regions operatively linked with a heterologous gene of interest, as well as plant cell cultures and transgenic plants comprising these vectors. A method for the preparation of a plant using the regulatory regions of this invention are also disclosed.

Regulatory region of a lipid transfer protein (LtpW1) from aleuron tissue of wheat

is a plant pathogen, attacking a wide range of plant species including corn (ear and stalk rot), barley, and wheat (head blight).epidemics result in millions of dollars of losses in crop revenues.infection in the cereals reduces both grain yield and quality. Mycotoxins are produced by many fungalspecies and thus the grain becomes contaminated with these mycotoxins, such as the trichothecenes. The major trichothecene produced byis deoxynivalenol (abbreviated as DON, also known as vomitoxin). Trichothecenes are potent protein synthesis inhibitors and are quite toxic to humans and livestock. A yeast gene has been identified which confers upon yeast tolerant of the trichothecene, trichodermin. A corresponding plant gene has been prepared, which has been used to transform plants. These transformed plants have an increased resistance toinfestation.

Tolerance of trichothecene mycotoxins in plants through the modification of the ribosomal protein L3 gene

This invention is directed to a regulatory region obtained from a wheat aleurone gene LtpW1. This regulatory region, or truncated derivatives of this regulatory region, can be used to express heterologous genes of interest within aleurone cells of a plant. Furthermore, this invention is directed to a truncated LtpW1 regulatory region that exhibits constructive activity within both monocot and dicot plants. This invention is also directed to vectors comprising these regulatory regions operatively linked with a heterologous gene of interest, as well as plant cell cultures and transgenic plants comprising these vectors. A method for the preparation of a plant using the regulatory regions of this invention are also disclosed.

Wheat aleurone regulatory region

Fusarium graminearum is a plant pathogen, attacking a wide range of plant species including corn (ear and stalk rot), barley, and wheat (head blight). Fusarium epidemics result in millions of dollars of loses in crop revenues. Fusarium graminearum infection in the cereals reduces both grain yield and quality. Mycotoxins are produced by many fungal Fusarium species and thus the grain becomes contaminated with these mycotoxins, such as the trichothecenes. The major trichothecene produced by F. graminearum is deoxynivalenol (abbreviated as DON, also known as vomitoxin). Trichothecenes are potent protein synthesis inhibitors and are quite toxic to humans and livestock. A yeast gene has been identified which is resistant to the trichothecene, trichodermin. A corresponding plant gene has been prepared, which has been used to transform plants and would be suitable to transform animals. These transformed plants have an increased resistance to Fusarium infestation. Potentially, transformed animals could have an increased tolerance to the trichothecene mycotoxins.

Tolerance of trichothecene mycotoxins in plants and animals through the

This invention is directed to regulatory elements obtained from a wheat aleurone gene LtpW1. The regulatory element, analogs thereof, or truncated derivatives of this regulatory element, can be used to express heterologous genes of interest within the aleurone cells of a plant. This invention is also directed to vectors comprising these regulatory elements operatively linked with a heterologous gene of interest, as well as plant cell cultures and transgenic plants comprising these vectors. A method for the preparation of a plant using the regulatory elements of this invention are also disclosed. Furthermore, this invention is directed to a truncated LtpW1 regulatory element that exhibits constitutive activity within plants.

Wheat aleurone regulatory elements

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Greely, Canada

Linda J Harris