Giner, Inc.

Giner Life Sciences, Inc.

Linda A Tempelman

Simon G Stone

Klearchos K Papas

Anthony B LaConti

Mourad Manoukian

John Forchione

Monjid Hamdan

W Michael Krebs

John W Forchione, Jr

Melissa Schwenk

William Earl Scott, Iii

Thomas M Suszynski

Shuichiro Matsumoto

Joana Ferrer Fabrega

Michael D Rizzari

Erich Muehlanger, Jr

Matthew P Steinbroner

System for gas treatment of cellular implants. The system enhances the viability and function of cellular implants, particularly those with high cellular density, for use in human or veterinary medicine. The system utilizes a miniaturized electrochemical gas generator subsystem that continuously supplies oxygen and/or hydrogen to cells within an implantable and immunoisolated cell containment subsystem to facilitate cell viability and function at high cellular density while minimizing overall implant size. The cell containment subsystem is equipped with features to allow gas delivery through porous tubing or gas-only permeable internal gas compartments within the implantable cell containment subsystem. Furthermore, the gas generator subsystem includes components that allow access to water for electrolysis while implanted, thereby promoting long-term implantability of the gas generator subsystem. An application of the system is a pancreatic islet (or pancreatic islet analogue) implant for treatment of Type 1 diabetes (T1D) that would be considered a bio-artificial pancreas.

System for gas treatment of a cell implant

Self-regulating electrolytic gas generator and implant system including the same. In one embodiment, the electrolytic gas generator is a water electrolyzer and includes a polymer electrolyte membrane with an anode on one side and a cathode on the other side. Anode and cathode seals surround the peripheries of the anode and cathode and include inlets for water and outlets for oxygen and hydrogen, respectively. A cathode current collector is placed in contact with the cathode, and an anode current collector, which may be an elastic, electrically-conductive diaphragm, is positioned proximate to the anode. The anode current collector is reversibly deformable between a first state in which it is in direct physical and electrical contact with the anode and a second state in which it distends, due to gas pressure generated at the anode, so that it is not in physical or electrical contact with the anode, causing electrolysis to cease.

Self-regulating electrolytic gas generator and implant system comprising the same

System for gas treatment of cellular implants. The system enhances the viability and function of cellular implants, particularly those with high cellular density, for use in human or veterinary medicine. The system utilizes a miniaturized electrochemical gas generator subsystem that continuously supplies oxygen and/or hydrogen to cells within an implantable and immunoisolated cell containment subsystem to facilitate cell viability and function at high cellular density while minimizing overall implant size. The cell containment subsystem is equipped with features to allow gas delivery through porous tubing or gas-only permeable internal gas compartments within the implantable cell containment subsystem. Furthermore, the gas generator subsystem includes components that allow access to water for electrolysis while implanted, thereby promoting long-term implantability of the gas generator subsystem. An application of the system is a pancreatic islet (or pancreatic islet analog) implant for treatment of Type 1 diabetes (T1D) that would be considered a bio-artificial pancreas.

Method and system for organ preservation. According to one aspect, an organ may be preserved by being perfused with an in situ generated preserving gas. The organ may be, for example, a human or porcine pancreas, and perfusion of the pancreas may be anterograde, retrograde, ductal, anterograde/ductal, or retrograde/ductal. The preserving gas used to perfuse the organ may be dissolved in a liquid and then administered to the organ as a gas/liquid solution or may be mixed with one or more other gases and then administered to the organ as a gas/gas mixture. The preserving gas may be, for example, oxygen gas generated in situ using an electrochemical oxygen concentrator. According to another aspect, an organ preservation system may include an electrochemical oxygen concentrator having a water vapor feed, as well as auxiliary equipment to control and measure delivery pressure, flow, temperature and humidity.

Perfusing an organ with an in situ generated gas

System for fluid perfusion of biological matter that includes tissue. According to one embodiment, the system may include a storage container for storing the biological matter, a thermal control device for cooling the contents of the storage container, a gas generator for generating a preserving gas, a fluid conduit coupled to the gas generator and insertable into tissue for delivering the preserving gas to the biological matter, and a process controller for controlling the operation of the gas generator and the thermal control device. The gas generator, in turn, may include an electrochemical oxygen concentrator and/or a water electrolyzer for generating the preserving gas. The system may further include a liquid perfusion system that includes a reservoir of liquid perfusate, a fluid delivery conduit for delivering liquid perfusate from the reservoir to the biological matter, and a fluid draining conduit for draining liquid perfusate from the biological matter.

System for fluid perfusion of biological matter comprising tissue

A multi-gas microsensor assembly for simultaneously detecting carbon dioxide and oxygen in real time. According to one embodiment, the assembly comprises a non-conductive, solid substrate. A plurality of sensing electrodes, a single reference electrode, and a single counter electrode are positioned on one side of the non-conductive, solid substrate. In addition, all of the electrodes are in intimate contact with the same side of a solid-polymer electrolyte anion-exchange membrane, the solid polymer electrolyte membrane having at least one gas diffusion opening aligned with each sensing electrode. The sensor is operated in a three-electrode potentiostatic mode, in which a constant potential is maintained between the sensing and reference electrodes, and the current is measured between the sensing and counter electrodes. Control of the electrodes is achieved with a small bi-potentiostat. The design of the bi-potentiostat allows at least two different sensing electrodes to share the same counter and reference electrodes.

Multi-gas microsensor assembly

A method for the detection of carbon dioxide gas using an electrochemical sensor. The method includes exposing a gas to a sensor, which includes a non-conductive solid substrate and at least one each of a metal oxide sensing electrode, a reference electrode and a counter electrode positioned on the substrate. A solid polymer electrolyte anion-exchange membrane is in intimate contact with the sensing electrode, reference electrode and counter electrode. The method is highly sensitive and selective to carbon dioxide with a very rapid response time.

Electrochemical carbon dioxide sensor

System for modifying the chemical composition of atmosphere within an enclosed space and incubator system including such a system. The concentration of oxygen within the enclosed space may be either increased or decreased using an electrochemical device. The concentration of carbon dioxide within the enclosed space may be increased using an electrochemical or chemical device. As necessary, purging of the system with ambient air can be a part of the process of controlling the chemical composition of the atmosphere. The present invention obviates the need to use pressurized gas cylinders to supply atmospheric gases to the enclosed space.

System for modifying the atmosphere within an enclosed space and incubator system including the same

An electrochemical sensor is provided for the detection of carbon dioxide gas. The sensor includes a non-conductive solid substrate and at least one each of a metal oxide sensing electrode, a reference electrode and a counter electrode positioned on the substrate. A solid polymer electrolyte anion-exchange membrane is in intimate contact with the sensing electrode, reference electrode and counter electrode. The sensor is highly sensitive and selective to carbon dioxide and has very rapid response time.

Growing community of inventors

Lincoln, MA, United States of America

Linda A Tempelman