USA As Represented by Secretary of the Navy

Linda A Lininger

Malford E Cullum

Lloyd Grant Simonson

Alan L McArthur

Sylvia Zottu Schade

James C Ragain, Jr

Paul Hine

Chun N Shih

Diane R Bienek

Sukjoon Park

The inventive subject matter relates to a method for detecting the presence of a biological substance of interest in a test sample of saliva or oral fluid, comprising combining said test sample with a fluorescence-labeled ligand to said biological substance and detecting a change in the fluorescence polarization of said test sample produced by binding of said fluorescence-labeled ligand to said biological substance. In one aspect of the inventive subject matter, said method comprises additional steps for comparing the fluorescence polarization of said test sample with the fluorescence polarization of a control solution. Also provided is a miniaturized, portable apparatus for measuring the fluorescence polarization of a liquid sample.

Fluorescence polarization instruments and methods for detection of exposure to biological materials by fluorescence polarization immunoassay of saliva, oral or bodily fluids

The inventive subject matter relates to a competitive method for estimating the concentration in a sample of aprotein or antibody thereof selected from the group consisting of protective antigen (PA), lethal factor (LF) and edema factor (EF). The method may employ the use of Fluorescence Polarization, FLT or FRET. The competitive methods are capable of detecting a target protein within 5 minutes within the range of 0.1 to 10.0 nM. The methods provide for the rapid detection and quantitation of bacteria, bacterial antigen or antibody in culture media or broth of growing cultures of bacteria, includingby fluorescent methods.

Rapid immunoassay of anthrax protective antigen in vaccine cultures and bodily fluids by fluorescence polarization

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Grayslake, IL, United States of America

Linda A Lininger