Pioneer Hi-bred International, Inc.

Verdia Inc.

Maxygen, Inc.

E.i. Dupont De Nemours and Company

E. I. Du Pont De Nemours

Codexis Mayflower Holdings, LLC

Pioneer Hi-bred Int'l Inc.

Roger Kemble

Christina Ivy

Billy Fred Mccutchen

Lorraine J. Giver

Nicholas B. Duck

Jeremy Minshull

Rebecca Gorton

Yong Hong Chen

Linda A Castle

Phillip A Patten

Jeremy S Minshull

Rebecca Lynne Gorton

Nicholas B Duck

Lorraine Joan Giver

Dan Siehl

Daniel Siehl

Cristina Ivy

Billy Fred McCutchen

Jon Edward Ness

Matthew B Tobin

Anthony Cox

Claus M Krebber

Willem P C Stemmer

Venkiteswatan Mani Subramanian

Henrik Albert

Claes Gustafsson

Robin A Emig

Sridhar Govindarajan

Yumin Tao

Richard John Fox

Ericka Bermudez

S Christopher Davis

Teddy Chen

Stephen DelCardayre

Ajoy K Roy

Gjalt W Huisman

Steven Henry Bass

Joseph A Affholter

Ying-Xin Zhang

Ling Yuan

Steve Bass

Jian Lu

William P C Stemmer

Yuxia Dong

Stephen delCardayre

Kevin E McBride

Andrew Duncan Satterfield

Venkiteswaran Subramanian

Matthew J Heckert

Theodore Mitchell Klein

Zhenglin Hou

Steven Gutteridge

Jingtong Hou

Sean M Bertain

Kay Walter

Hyeon-Je Cho

York Moy

Takashi Yamamoto

Tai Minh

John Lawrence Andreassi, Ii

Tony Cox

Daniel L Siehl

Sridar Govindarajan

Ajoy Apt120 Roy

Phil Patten

Rebecca Lynne Apt819 Gorton

Christopher S Davis

Lorraine J Giver

Compositions and methods comprising polynucleotides and polypeptides having glyphosate-N-acetyltransferase (GLYAT) activity are provided. In specific embodiments, the sequence has an improved property, such as, but not limited to, an improved specificity for glyphosate when compared to an appropriate control resulting in decreased off target acetylation of, e.g. an amino acid such as aspartate. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the GLYAT sequences. Various methods of employing the GLYAT sequences are provided. Such methods include methods for producing a glyphosate tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.

Glyphosate-n-acetyltransferase (GLYAT) sequences and methods of use

Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.

Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (HPPD) activity

Glyphosate-N-acetyltransferase (GLYAT) sequences and methods of use

Compositions and methods comprising polynucleotides and polypeptides having ALS activity and tolerance to at least one ALS inhibitor are provided. In specific embodiments, the sequence has an increased preference for 2-ketobutyrate, when compared to an appropriate control, such as for example, HRA, and/or a preference for 2-ketobutyrate similar to a native ALS. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the ALS inhibitor tolerant sequences. Various methods of employing the ALS inhibitor tolerant sequences are provided. Such methods include methods for producing an ALS inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.

Methods and compositions involving ALS variants with native substrate preference

The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention additionally provides digital systems and software for performing these methods.

Methods, systems, and software for identifying functional bio-molecules

Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO:1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof. Cells, plant cells, plants and seeds are further provided which comprise such sequences. Methods of use of the various sequences are also provided.

Methods and compositions for targeting sequences of interest to the chloroplast

Compositions and methods comprising promoters from the 4-hydroxyphenylpyruvate dioxygenase (HPPD) gene and active variants and fragments thereof, as well as chimeric promoters employing regulatory regions of the HPPD promoters are provided. Further provided are expression cassettes and plants comprising the various promoters disclosed herein operably linked to a polynucleotide of interest. Methods employing the various promoters described herein to modulate the expression of polynucleotides of interest are further provided.

Chimeric promoters and methods of use

Compositions and methods are provided for the efficient transformation of a dicot plant. More particularly, compositions and methods of the present invention find use in agriculture for-mediated transformation of a dicotyledonous plant. The compositions include cultivation media comprising high concentrations of sucrose and glucose. The cultivation media find use in methods directed to-mediated transformation of a dicot plant with a gene of interest. In this manner, any gene of interest can be introduced into a dicot plant with high transformation efficiency and reduced tissue necrosis.

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Methods, systems, and software for identifying functional biomolecules

The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

Evolution of whole cells and organisms by recursive sequence recombination

Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

Glyphosate-N-acetyltransferase (GAT) genes

Proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are polynucleotides capable of encoding these proteins, compositions that include one or more of these proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these compounds, diversification methods involving the compounds, and methods of using the compounds. Some of the methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate.

Growing community of inventors

Mountain View, CA, United States of America

Linda A Castle