Ultra-microrigin Biomedical Technology Co., Ltd.

Other

Gwo Xi Stem Cell Applied Technology Co., Ltd.

Super Well Biotechnology Corporation

Lin-Hsiang Chuang

Ming-Hsi Chuang

Chu-Ting Liu

I-Lung Yu

Zer-Ran Yu

Chiu-Ying Peng

Be-Jen Wang

Chi-An Li

Yun-Chen Lin

Shuen-Ane Kang

Shu-Mei Lin

Cheng-Yu Chi

Chi-Hsuan Chuang

Chu-Ting Lin

Hua-Ching Lin

Hsin-Yu Chung

Ming-Hsuan Lu

Wen-Peng Shen

The present invention relates to a method for synthesizing artepillin C and a synthetic method for synthesizing an intermediate compound for use in preparing artepillin C. The structural formula of artepillin C is shown in the following formula (IV), and the intermediate compound is shown in the following formula (II). The synthesis method includes the following steps: a first reaction step: in the presence of a base initiator, a compound A shown in formula I and 3,3-dimethylallyl bromide are mixed, the reaction continues for a first reaction time and is stopped to generate an intermediate of artepillin C shown in formula II; and a second reaction step: the intermediate of artepillin C is dissolved in an aqueous alcohol solution and then mixed with an alkali metal salt, and the reaction continues for a second reaction time to generate artepillin C.

Method for synthesizing artepillin C and intermediate compound thereof

A method of culturingwith high Triterpenoids includes mixing a malt extract, glucose, peptone, agar, and a mushroom's extract with distilled water to obtain a culture medium; providing the culture medium to a high-pressure sterilization machine for sterilization to obtain a sterilized culture medium; providing the sterilized culture medium to an incubator in a disinfection environment to obtain a solidified culture medium after a predetermined time; transplantingstrains to the solidified culture medium at separated positions; and exposing the solidified culture medium under a beam.

[object Object]

A method of using supercritical fluid technology to separate and purify the functional components ofis described. Triterpenoids and polysaccharides ofare extracted from a fractionation tank containing extract ofand supercritical solvent at a set temperature and pressure. The extracted functional components and supercritical solvent are then continuously fed into three separating tanks at a set temperature and a pressure below that of the aforesaid pressure to obtain various functional components of triterpenoids and polysaccharides from thefeed in each separating tank.

Method of using supercritical fluid technology to separate and purify functional components of Antrodia camphorata

The present invention provides a method of separating estrogen from placenta, which uses supercritical fluid technique to load the placenta powder of human body or sheep, pig, deer and other animals into an extraction tank. Under the operating conditions of preset pressure and temperature, supercritical solvent is added into the extraction tank to extract estrogen from placenta, so as to acquire de-estrogen placenta powder and placenta extract liquor. Under the same condition, the de-estrogen placenta extract liquor and supercritical solvent are added by a preset volume flow ratio into an adsorption tank. The estrogen in the placenta extract liquor is adsorbed by the adsorption tank to obtain de-estrogen placenta extract. It is then eluted with ethanol solution by gradient proportion to obtain purified natural estrogen.

Method for separating estrogen from placenta

A method of making, separating, and purifying artepillin C in propolis includes mixing propolis with ethanol to obtain a propolis-ethanol extract; providing supercritical carbon dioxide and the propolis-ethanol extract to a chromatographic column to separate wax, artepillin C, and flavonoids; providing supercritical carbon dioxide, the artepillin C, and the flavonoids to an adsorption column to remove the flavonoids; keeping the adsorption column still; and providing ethanol to the adsorption column to obtain the purified artepillin C.

Device method of making, separating, and purifying artepillin C in propolis

Growing community of inventors

Zhubei, Taiwan

Lin-Hsiang Chuang