Bgi Shenzhen

Qingdao Mgi Tech Co., Ltd

Shenzhen Bang-bell Electronics Co., Ltd.

Lin He

Sha Liao

Jian Li

Wenwei Zhang

Ao Chen

Hanjie Shen

Junqiang Xu

Shengyuan Li

Provided are a rolling circle amplification method, a method for preparing a sequencing library, and a DNA nanoball prepared therefrom. The rolling circle amplification method includes: sequentially denaturing and annealing a double-stranded DNA and a mediating sequence in a same system, to complementarily pair the mediating sequence with two ends of a denatured single-stranded DNA; simultaneously introducing a ligase and a polymerase into the system to connect the two ends of the single-stranded DNA under action of the ligase; and performing a rolling circle amplification reaction under action of the polymerase by using the mediating sequence as a primer and the single-stranded DNA as a template, to obtain DNA nanoball.

Rolling circle amplification method, method for preparing sequencing library, and DNA nanosphere prepared therefrom

Provided is a method for sequencing a long-fragment nucleic acid. The nucleic acid molecules each containing a long insert, a first sequencing adapter, and a second sequencing adapter, is used to construct a sequencing library, and the sequencing is performed in segments to sequence the nucleic acids having the long inserts.

Method for sequencing long-fragment nucleic acid

Desktop light therapy device

Light therapy device

LED light source module

Growing community of inventors

Guangdong, China

Lin He