Massachusetts Institute of Technology

Lijuan Zhang

Robert D Rosenberg

Jian Liu

John J Schwartz

David L Beeler

Nicholas W Shworak

Linda M S Fritze

Disclosed are methods of 6-O-sulfating glucosaminyl N-acetylglucosamine residues (GlcNAc) in a polysaccharide preparation and methods of converting anticoagulant-inactive heparan sulfate to anticoagulant-active heparan sulfate and substantially pure polysaccharide preparations may by such methods. Also disclosed is a mutant CHO cell which hyper-produces anticoagulant-active heparan sulfate. Methods for elucidating the sequence of activity of enzymes in a biosynthetic pathway are provided.

6-O sulfated polysaccharides and methods of preparation thereof

Disclosed are novel isolated nucleic acids and substantially pure protein preparations for naturally occurring and synthetic or chimeric heparan sulfate D-glicosaminyl 3-O-sulfo-transferases (3-OSTs). Also disclosed are uses for these genes and proteins, including uses for the modification and sequencing of glycosaminoglycans.

Heparan sulfate D-glucosaminyl 3-O-sulfotransferases, and uses therefor

Growing community of inventors

St. Charles, MO, United States of America

Lijuan Zhang