Shenzhen Mindray Bio-Medical Electronics Co., Ltd.

Chinese Academy of Sciences

Southern University of Science and Technology

Lihua Zhou

Feng Li

Zhihong Wang

Yanwen Weng

Ping Gong

Jinhong Qiu

Keith Man Chung Wong

Chuangjun Liu

Lintao Cai

Through the use of a rhodamine appended chelate, a versatile strategy has been demonstrated to generate mitochondria-targeting photosensitizers via the incorporation of variety of luminescent transition metal systems. The generation of triplet excited state of rhodamine moiety endows the complexes with mitochondria-targeting photosensitizing ability to form singlet oxygen (O) for use as photodynamic therapy (PDT) agent. The combination of rhodamine organic dye and luminescent transition metal centers in such hybrid systems exhibits the synergistic merits, including low dark cytotoxicity, selective tumor cell uptake, high molar absorptivity for low-energy excitation in the visible region, and high photostability.

Rhodamine triplet state complex and preparation and photodynamic therapy (PDT) study thereof

Various embodiments disclose a colorimetric absorbance measurement method and a system for performing the same. The method comprises driving a reaction tray carrying a plurality of reaction cuvettes to rotate at a speed; transferring or rotating filters on a filter wheel to a light path of a light beam, starting from a filter of a first wavelength; and sampling photoelectric data when the light beam has passed through each reaction cuvette in some embodiments. The method ensures consistency in the measurement and synchronization between the calibration and the sample test and reliability of the measurements. Various embodiments simplify data processing and reduce the complexity of the system.

Colorimetric absorbance measurement method, apparatus and system

A spectrophotometric optical system of a microplate reader and a filter wheel thereof are disclosed. The filter wheel comprises a pivotable wheel body, at least one narrow-band filter with a relatively long central wavelength mounted to the wheel body, and at least one narrow-band filter with a relatively short central wavelength mounted to the wheel body. The narrow-band filter with a relatively long central wavelength is provided with a diaphragm on a front surface thereof The diaphragm is formed with a plurality of apertures. By the diaphragm attached to the front surface of the narrow-band filter with a relatively long central wavelength, an energy matching between light of longer wavelength and light of shorter wavelength may be achieved. Further, due to the uniformly distributed apertures in the diaphragm, an even light spot may be obtained.

Spectrophotometric optical system of microplate reader and filter wheel thereof

Growing community of inventors

Shenzhen, China

Lihua Zhou