Chinese Academy of Sciences

Lihua Zhang

Yukui Zhang

Kaiguang Yang

Yuan Zhou

Haibin Luo

Long Wang

Wenge Zheng

Zhigang Sui

Qian Ren

Jianhui Liu

Minghui Wu

Huiming Yuan

Fei Wu

Gun cleaning case

The present application relates to a supercritical fluid injection foaming polylactide foam material and a preparation method therefor. The method includes: first obtaining a surface-modified cellulose nanofiber aqueous solution; then melting and blending the cellulose nanofiber aqueous solution and a polylactide twice; passing same through extrusion, cooling under water, and granulation so as to obtain a polylactide/cellulose nanofiber composite material; then plasticizing and melting the polylactide/cellulose nanofiber composite material in a microporous foaming injection molding machine; uniformly mixing same with a supercritical fluid foaming agent in the injection molding machine; injecting same into a mold cavity; and subjecting the resultant to post-treatment so as to obtain a polylactide foam material. The polylactide foam material has a sandwich structure, in which two outer surface layers are solid layers that do not contain any foam, and the sandwiched layer is a foam layer having a cellular structure.

Supercritical fluid injection foaming polylactide foam material and preparation method therefor

The invention relates to a method for the treatment of body fluid proteins, by which proteins from body fluids such as blood or urine are extracted by adding a certain proportion of high molecular polymer solution under low temperature condition followed by denaturation and reduction by adding a certain concentration of surfactant and tris(2-carboxyethyl) phosphine (TCEP) under a high temperature condition. Subsequently, the iodoacetic acid brushes grafted on silica microspheres called as solid-phase alkylation reagents are added into protein solution, which can react rapidly with the protein sulfhydryl group. After centrifugation, the microspheres are obtained and repeatedly washed with methanol and buffer to remove interferences such as sugars, salts, surfactants, lipids to obtain high-purity proteins, and finally protease is added to digest proteins into peptides. After centrifugation, the peptide products are obtained, and directly analyzed by liquid chromatography-mass spectrometry (LC-MS) system. Compared with the traditional protein pretreatment method, the method has many advantages such as good anti-interference capability, easy operation and short pretreatment time.

Method for pretreating protein in ex vivo body fluid

A multiplex proteome quantification method based on isobaric dimethyl labeling implements dimethyl labeling of peptide N-terminal in an acidic condition and C-terminal in an alkaline condition one after another by means of Hall the property that a dimethylation reaction has different rates on an amino group at the peptide N-terminal and an amino group on a Lysine side chain at the peptide C-terminal in the acidic condition. Multiplex labeling of peptide samples is implemented by means of the organic combination of various isotope forms of a dimethyl labeling reagents. The mass-to-charge ratios in MS1 of peptides after multiplex labeling are completely the same, the mass-to-charge ratios of the fragment ions in MS2 are different, and multiplex quantitative analyses are carried out by extracting the intensity values of corresponding fragment ions in the MS2.

Multiplex proteome quantification method based on isobaric dimethyl labeling

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Liaoning, China

Lihua Zhang