Abbvie Inc.

Lihua Yang

Natarajan Ramasubramanyan

Hong Yang

Christopher Chumsae

Matthew Omon Herigstad

Zehra Kaymakcalan

Xiaobei Zeng

Kartik Subramanian

Wen Chung Lim

Kathreen A Gifford

Diane D Dong

The instant invention relates to the field of protein production and purification, and in particular to compositions and processes for controlling the amount of charge variants, aggregates, and fragments of a protein of interest, as well as host cell proteins, present in purified preparations by applying particular chromatography conditions during such protein purification.

Protein purification methods to reduce acidic species

The instant invention relates to low acidic species (AR) compositions comprising a protein, e.g., an antibody, or antigen-binding portion thereof, and methods, e.g., cell culture and/or protein purification methods, for producing such low AR compositions. Methods for using such compositions to treat a disorder, e.g., a disorder in which TNF&#x3b1; is detrimental, are also provided.

Low acidic species compositions and methods for producing and using the same

Low acidic species compositions

The instant invention relates to low acidic species (AR) compositions comprising a protein, e.g., an antibody, or antigen-binding portion thereof, and methods, e.g., cell culture and/or protein purification methods, for producing such low AR compositions. Methods for using such compositions to treat a disorder, e.g., a disorder in which TNF&#x3b1; is detrimxental, are also provided.

The present invention relates to compositions and highly sensitive methods for the detection of biotechnology product residual when monitoring product carry over and/or for cleaning verification in the manufacture of biotechnology products. In particular, the present invention is directed to immunoassays wherein one or more capture antibodies, or antigen binding fragments thereof, are used to detect residuals associated with the production of biotechnology products.

Highly sensitive monoclonal antibody residual detection assay

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Westborough, MA, United States of America

Lihua Yang