Jiangnan University

Changde Yandi Biotechnology Limited Company

Lihua Hou

Min Zhang

Sifu Yi

Wuxiong Yang

Xiaoling Guo

Hao Shi

Zhongqin Li

Yanjun An

Yanyan Lao

The present application belongs to the technical field of research and development of fermented beverages, and a method for preparingferment by two-stage fermentation and complex enzymatic hydrolysis is provided. The present application involves preparation of apowder and a Cordyceps militaris slurry, preparation of afermentation substrate, lactic acid bacteria fermentation in combination with complex enzymatic hydrolysis, and yeast fermentation, followed by centrifugation, filtration, formulation, sterilization, and filling to obtain a product. By employing lactic acid bacteria fermentation in combination with complex enzymatic hydrolysis, and secondary fermentation by yeast, the present application can quickly finish a whole fermentation process in 2 to 3 days.

Method for preparing Cordyceps militaris ferment by two-stage fermentation and complex enzymatic hydrolysis

Disclosed is an ultrasonic composite acidic water extraction method for apolysaccharide and cordycepin in, which falls within the technical field of food processing. The method comprises: picking, washing, drying, pulverizing and degreasingfruiting bodies to obtain adry powder; then immersing same in a prepared diluted hydrochloric acid solution to perform three cycles of ultra-low temperature freezing and microwave defrosting, and at the same time using low-frequency ultrasonic waves to carry out assisted stirring and extraction; then subjecting an extract to evaporation and concentration treatments; and finally, performing freeze-drying on the extract to collect a dry powder of a water extraction product. The amount of an additive used in the method meets domestic and foreign usage requirements of food additives, and the operation is simple, practical and uses a combination of chemical and physical methods, thereby saving time and being highly efficient.

Ultrasonic composite acidic water extraction method for cordyceps polysaccharide and cordycepin in

A method for preparingnano-composite powder with protein-polysaccharide complex coacervation stability. The method includes sorting, drying, crushing suspendingultrafine powder, emulsifying primarily, re-emulsifying, adjusting pH value and conducting spray drying. The emulsion stability after primary emulsification is not high, so re-emulsification technology is increased, and lactalbumin with higher emulsifying property and higher nutritional value are adopted as enhanced emulsifier, thus enhancing stability of an emulsifying system. The network envelopment stability of polysaccharide colloidal xanthan gum or kudzu powder is used to consolidate the stability of the emulsifying system, and a Pickering emulsifying system with multilayer of solid particle stability is constructed. The system has high stability and can effectively and organically combine fully pulverized components ofinto microcapsule composite powder, and the powder has good dispersity and has higher degree of homogenization after being dissolved in the water.

Method for preparing cordyceps militaris nano-composite powder with protein-polysaccharide complex coacervation stability

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Jiangsu, China

Lihua Hou