Chinese Academy of Sciences

Lianyan Wang

Wei Wei

Qiang Wei

Guanghui Ma

Zhiguo Su

A method of fluorescence imaging is disclosed that uses a non-labeled fluorescent microsphere comprising a primary amine polymer, wherein the microsphere does not contain an additional fluorescent substance, and wherein at least one primary amine group in the primary amine polymer has the structure of &#x2014;N&#x2550;R&#x2014;, in which R is a residue after the reaction of an aldehyde-type cross-linking substance, and the other primary amine groups have the structure of &#x2014;NH. The fluorescence color of the microspheres can be adjusted by selecting different aldehyde-type cross-linking substances or combinations, and the fluorescence intensity of the microspheres can be controlled not only by the addition of the aldehyde-type cross-linking substances and the time of the cross-linking reaction, but also by adjusting the diameters of the microspheres.

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Beijing, China

Lianyan Wang