Dsm IP Assets B.v.

Genomatica, Inc.

Liang Wu

Jan Metske Van Der Laan

Viktor Marius Boer

Robbertus Antonius Damveld

Cornelis Maria Jacobus Sagt

Marco Alexander Van Den Berg

Axel Christoph Trefzer

Stefaan Marie Andre De Wildeman

René Verwaal

Alrik Pieter Los

Johannes Andries Roubos

Priscilla Zwartjens

Bernard Meijrink

Petronella Catharina Raemakers-Franken

Herman Jan Pel

Rene Verwaal

Lucie Parenicova

Noel Nicolaas Maria Elisabeth Van Peij

Aaron Adriaan Winkler

Adam G Lawrence

Ulrike Maria Mueller

Jan Herman Pel

Lourina Madeleine Raamsdonk

Jan Metske Laan Van Der

Hendrik Jan Noorman

Martin Lehmann

Cornelis Haringa

Stefaan Marie André De Wildeman

Noël Nicolaas Maria Elisabeth Peij Van

Manoj Kumar

Joshua Truehart

The present invention relates to a method for preparing an adipate ester or thioester. The invention further relates to a method for preparing adipic acid from said ester or thioester. Further the invention provides a number of methods for preparing an intermediate for said ester or thioester. Further the invention relates to a method for preparing 6-amino caproic acid (6-ACA), a method for preparing 5-formyl valeric acid (5-FVA), and a method for preparing caprolactam. Further, the invention relates to a host cell for use in a method according to the invention.

Adipate (ester or thioester) synthesis

The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.

Acetyl-CoA producing enzymes in yeast

The present invention relates to a recombinant eukaryotic cell selected from a yeast of a filamentous fungus comprising a nucleotide sequence encoding a NAD(H)-dependent fumarate reductase that catalyzes the conversion of fumaric acid to succinic acid. The invention further relates to a process for the production of succinic acid wherein the eukaryotic cell according to the present invention is used.

Succinic acid production in a eukaryotic cell

The present invention relates to a recombinant yeast comprising a nucleotide sequence encoding a heterologous enzyme that catalyses the conversion of malic acid to fumaric acid. The invention further relates to a process for the production of a dicarboxylic acid wherein the yeast according to the present invention is used.

Dicarboxylic acid production in a recombinant yeast

The present invention relates to a recombinant eukaryotic microbial cell comprising a nucleotide sequence encoding a heterologous enzyme catalyzing the conversion from phosphoenolpyruvate to oxaloacetate whereby ATP is generated. The invention further relates to a process for the preparation of a dicarboxylic acid such as succinic acid and fumaric acid, comprising fermenting the eukaryotic microbial cell according to the invention in a suitable fermentation medium.

Dicarboxylic acid production in eukaryotes

The present invention relates to a method for improving protein yield. The method comprises modifying the value of a set of relevant protein features to fall within an optimal range or to become more close to an optimal value for one or more protein features in the eukaryotic host.

Method for improving the yield of a polypeptide

The present invention relates to a novel method for the preparation of 1,4-diaminobutane [DAB]. The method according to the present invention involves at least one biocatalytic step which comprises the biocatalytic production of at least one N-protected precursor of DAB. The present invention also relates to a method for the preparation of DAB involving at least one biocatalytic step, and comprising the steps of a) biocatalytically preparing an N-protected precursor of DAB yielding a&#x2014;biocatalytic reaction mixture containing the N-protected precursor of DAB, b) recovering the N-protected precursor from the biocatalytic reaction mixture and c) converting the N-protected precursor into DAB. More in particular, the present invention relates to a method for the preparation of DAB, wherein the at least N-protected precursor of DAB is selected from the group consisting of N5-protected ornithine, N-protected DAB, and N-protected 4-aminobutyraldehyde.

Preparation of 1,4-diaminobutane

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Delft, Netherlands

Liang Wu