Hubei University

Li Yi

Yu Wang

Lixin Ma

Shihui Yang

Wei Shen

Runxia Li

Yongfu Yang

Ju Huang

A method for identifying and characterizing biological parts based on omics datasets and a dual-fluorescent reporter gene system, and a biological part library constructed thereon are provided, relating to a technical filed of biology. The method includes steps of: identifying the biological parts using the omics datasets; constructing a single-fluorescent reporter gene system using a shuttle vector pEZ15Asp as a skeleton for screening and determining fluorescent reporter genes; obtaining a dual-fluorescent reporter gene system skeleton; constructing recombinant plasmids, and finally transforming into competent cells for quantitative analysis of fluorescence intensities. The present invention is convenient and quick, and can screen and identify different biological parts such as RBS, UTRs, promoters, and terminators of different intensities in batch quantitatively in a relatively short time. Moreover, the present invention can quickly expand the biological part library of, so as to be applied in metabolic engineering of different demands.

Method for characterizing biological part based on dual-fluorescent reporter gene system and biological part library constructed thereon

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Hubei, China

Li Yi