Jiangsu Academy of Agricultural Sciences

Nanjing University of Aeronautics and Astronautics

Nanjing Hydraulic Research Institute

Guotai (taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou

Li Wang

Qiyan Xiong

Zhixin Feng

Maojun Liu

Yuzi Wu

Yanna Wei

Zhanwei Wang

Qiuwen Chen

Cheng Chen

Yuqing Lin

Liuming Hu

Tiesheng Guan

Guoqing Shao

Beibei Liu

Yun Bai

Yanfei Yu

Yuan Gan

Jia Wang

Daohua Zhang

Dongxia Liu

Guoqinq Shao

Lizhong Hua

Shouwen Ren

Xuemin Wang

Xiaomin Fang

Bixia Li

Yanfeng Fu

Weimin Zhao

Xiaobo Xu

Haiyan Wang

Guotai Wu

Feng Tu

Ranran Feng

Fangfang Bai

Weijia Chen

A composition and a kit for detectingare provided. The composition for detectingis an aqueous solution including a primer M-F, a primer M-R, and a probe M-P. A sequence of the M-F is shown in SEQ ID NO: 1. A sequence of the M-R is shown in SEQ ID NO: 2. A nucleotide sequence of the probe M-P is shown in SEQ ID NO: 3, and includes a fluorophore FAM linked at a 5' terminus and a quencher BHQ1 linked at a 3′ terminus. The composition exhibits high sensitivity, strong specificity, and a wide detection range when used in the detection of

Composition and kit for detecting mycoplasma

A method for implementing fault diagnosis by means of a spread spectrum carrier includes the following steps: designing incident signal parameters, selecting a spread spectrum sequence for fault detection, determining a center frequency and a sequence length of a spread spectrum code, and segmenting and transforming a power carrier source signal; using the fault detection spread spectrum sequence as a carrier spread spectrum code, and performing spread spectrum modulation on the transformed power carrier source signal to generate an SSPLCR sequence; coupling the SSPLCR sequence to a cable to be tested, and when the cable works normally without failure, transmitting the SSPLCR signal to the receiving terminal via the cable; when the cable fails, reflecting the SSPLCR signal back to the transmitting terminal.

Method for implementing fault diagnosis by means of spread spectrum carrier

A pig production method by using a detachable and washable wet curtain for intensive pig farming The detachable and washable wet curtain includes a wet curtain mounting frame (), a spray tube (), a plurality of rotatable reels () equivalent to sleeves, a reel () with a buckle, a wet curtain net (), a reel () with a buckle, and a manual handle (). The wet curtain is formed by a low-cost common material, namely a plastic net and the like, is convenient to mount, detach, and wash, and is not prone to making dust, scale, algae, mosquitos, and the like accumulate thereon, thus reducing the replacement cost and use cost. Therefore, a new cooling method based on the wet curtain is provided for pig farms in summer, and it is also suitable for other livestock farms in summer.

Pig production method by using detachable and washable wet curtain

A method for controlling the gate based on the habitat requirement for fish overwintering in rivers. According to the characteristics of biological habitat of the river and the habitat demand of fishes during overwintering, the method specifically comprises the steps of: firstly, determining candidate fishes for ecological flow calculation though fish resources investigating and historical data, and then screening out the target fish by adopting hierarchical analysis method; Secondly, establishing a quantitative response relationship curve between target fish physiological adaptions and water temperature, obtaining ecological water level which ensures the target fish overwintering safely according to the vertical temperature distribution, and establishing the relation between water depth and discharge using hydrodynamic model; finally, setting up a gate control system including a radar water level meter in the overwintering areas and an ecological water level management system in a gate control room.

Method for controlling the gate based on the habitat requirement for fish overwintering in rives

Disclosed are an attenuated live vaccine against mycoplasmal pneumonia of swine (MPS) and use thereof. In the present invention, pathological lung tissues of swine having typical(Mhp) infection and no obvious other pathogenic infections are screened, and subcultured 100 generations in lungs of newborn rabbits; then, Mhp strains are isolated and serially subcultured in a medium; and the Mhp strain AN306 is obtained by screening a plurality of strains, which is deposited with an accession number: CCTCC NO. M2012431. Also disclosed is a live vaccine formulation against MPS prepared on the basis of the attenuated strain and comprising live attenuated strain, a pharmaceutically acceptable carrier or excipient, and optionally an adjuvant and immunogens of other pathogens.

Attenuated live vaccine against mycoplasmal pneumonia of swine (MPS) and use thereof

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Nanjing, China

Li Wang