Jiangnan University

Li Sun

Jian Chen

Guocheng Du

Jianghua Li

Long Liu

Xueqin Lv

The present invention provides an acid-tolerantstrain and use thereof. By using exogenously added malic acid as a stress, an acid-tolerant mutantstrain MTPfo-4 is obtained by directed evolution screening in the laboratory, which tolerates a minimum pH of 2.44. The mutant strain MTPfo-4, tolerant to multiple organic acids, has an increased tolerance to exogenous malic acid of up to 86.6 g/L. The mutant strain MTPfo-4 obtained is further identified. The mutant strain grows stably and well, and can tolerate a variety of organic acids (lactic acid, malic acid, succinic acid, fumaric acid, citric acid, gluconic acid, and tartaric acid). It also has a strong tolerance to inorganic acids (HCl and HPO). This is difficult to achieve in the existing research and reports of. The strain is intended to be used as an acid-tolerant chassis cell factory for producing various short-chain organic acids.

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Wuxi, China

Li Sun