Amoy Diagnostics Co., Ltd.

Xiamen Amoy Diagnostics Co., Ltd.

Li Ruan

Xuchao Li

Huijuan Ge

Baolei Jin

Yan Biao Chen

Weijie Shi

Limou Zheng

Linhua Zhang

Ning Chen

Qinghua Lin

Donghua He

Zhenghua Tang

Binfeng Ji

Yan Chen

The present invention discloses a probe applicable to high-throughput sequencing and a method of enriching a target region using the probe. The probe includes, in an order from a 5' end, a ligation arm, a masking sequence 1, a UID sequence 1, an Illumina Tag1 sequence, a dU region, an Illumina Tag2 sequence, a UID sequence 2, a masking sequence 2 and an extension arm; wherein the masking sequence 1, the masking sequence 2, the dU region, the UID sequence 1, and the UID sequence 2 may be 0 bp. When sequencing is performed by using a library built by the probe of the present invention, it is not necessary to add a sequencing primer additionally, and adopting the method of the present invention may directly use an RNA as a template to capture a target region, so as to simplify the experiment procedure.

Probe and method of enriching target region applicable to high-throughput sequencing using the same

A multi-position double-tag adapter set for detecting gene mutation and preparation method therefor and application thereof, the multi-position double-tag adapter set comprising a double-tag adapter A, a double-tag adapter B and a double-tag adapter C. The double-tag adapter A, the double-tag adapter B and the double-tag adapter C are obtained respectively by hybridizing an adapter primer P5 with an adapter primer P7-A, an adapter primer P7-B and an adapter primer P7-C 5' ends of which are all modified with biotin. Using the multi-position double-tag adapter set, the mutation rate of 1&#xd7;10genes may be accurately detected and the sensitivity of gene mutation detection may be effectively improved. A plurality of mutation sites of a plurality of genes may be detected by one-time sequencing in combination with throughput of high-throughput sequencing.

Multi-position double-tag connector set for detecting gene mutation and preparation method therefor and application thereof

Loop-shaped primer used in nucleic acid amplification is an oligonucleotide with 3-20 bases in both 3' and 5&#x2032; ends which can be combined together to form a double-strand under appropriate conditions, resulting in the primer forming a stem-loop structure. The double-stranded structure is opened and the stem-loop structure dissolves when the primer recognizes and hybridizes with the target sequence. If the target sequence is not present the primer can form a stem-loop structure automatically by self-annealing. The primer can comprise a universal tag sequence or not. Together with universal tag sequence primer the primer comprising the universal tag sequence can be used for a second round of amplification. The primer has high specificity and does not form a primer dimmer. The primer is easy to design and is suitable for measuring gene expression and detecting features of nucleic acids such as SNPs and rare mutations.

Loop-shaped primer used in nucleic acid amplification and the use thereof

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Xiamen, China

Li Ruan