Food Industry Research and Development Institute

Li-Ling Liaw

Gwo-Fang Yuan

Ing-Er Hwang

Ching-Ping Tseng

Chung-Tsai Lee

Yi-Pei Chen

Chun-Lin Wang

I-Ching Chen

Yi-Hong Chen

Wen-Jung Wu

Hasiao Chi Peng

Ching-Yu Tu

Shu-Shan Liang

En-Hui Kuo

Yi-Ju Liu

Jyh-Wei Chen

Promoters and usage thereof. Two promoters, acuF and hsp promoters, comprise the nucleotide sequences of SEQ ID NO: 1 and 2, respectively.

Promoters and usage thereof

The present invention relates to an antifungal protein gene and cDNA sequence thereof, which is obtained by mining the whole genome sequences ofBCRC 38072 and the unigene database. The gene can encode an antifungal protein MAFP1. A purified protein obtained fromculture broth having molecular weight of about 7 kDa is identified as MAFP1 by N-terminal protein sequencing and comparative analysis. The purified MAFP1 protein can inhibit the growth of pathogens such asBCRC 33174 andBCRC 35004. In addition, it is found by PCR test that the gene of this antifungal protein exists in otherspecies such asand the like. It is also been proved that the mafp1 gene and cDNA thereof in fourstrains,(BCRC 38072, BCRC 38093 and BCRC 31502) andBCRC 31533, have the same DNA sequences.

Antifungal protein and usage thereof

The present invention relates to the cloning and expression of a novel &#x3b2;-agarase gene, agaB1, and a novel &#x3b2;-agarase, AgaB1, encoded from the gene. The present invention also provides a method for producing the &#x3b2;-agarase, and the utilization of the &#x3b2;-agarase.

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Cholesterol inhibitor produced by, monacolin k, is a secondary metabolite of polyketides. The invention provides probes specific to monacolin k biosynthesis gene cluster. BAC clones having putative monacolin k gene cluster were screened from BAC (bacterial artificial chromosome) library, and sequencing and annotation were performed on these clones. The results show that 2 polyketide synthase (PKS) genes and 7 regulatory genes related to monacolin k synthesis were obtained. Full-length cDNAs of these genes were then obtained by RT-PCR and cloned to expression vectors for the expression of these genes.

Monacolin K biosynthesis genes

Retrotransposons and methods related thereto. The retrotransposon comprises a nucleotide sequence selected from a group consisting of a nucleotide sequence of SEQ ID NO: 1, a nucleotide sequence of SEQ ID NO: 2, a nucleotide sequence of SEQ ID NO: 3, and a nucleotide sequence encoding a polypeptide of an amino acid sequence of SEQ ID NO: 4.

Fungi non-LTR retrotransposons and methods related thereto

Growing community of inventors

Hsinchu, Taiwan

Li-Ling Liaw