The United States of America, As Represented by the Secretary, Department of Health and Human Services

Johns Hopkins University

Li Lin

Edward G Lakatta

Wen Wei

Rui-Ping Xiao

Mark I Talan

Sungha Park

John Pang

The present disclosure provides a method for recombinant production of human sRAGE in mammalian cells, as well as a human sRAGE having a mammalian post-translational modification and compositions thereof. The present disclosure also provides a method of treating a vascular disease, injury, or inflammation in a mammal by administering to a mammal with a vascular disease, injury, or inflammation a composition comprising human sRAGE having a mammalian post-translational modification, thereby treating the vascular disease, injury, or inflammation in the mammal.

Human soluble receptor for advanced glycation end products (sRAGE), methods of preparing human sRAGE, and treatment methods using sRAGE

A series of p105-based NF-&#x3ba;B super repressors, designated p-105(sr), have been designed. The p105(sr), no longer generates p50 and undergoes signal-induced degradation, effectively inhibiting all NF-&#x3ba;B activities. Additionally, p105(sr) significantly enhances tumor necrosis factor alpha (TNF-&#x3b1;)-mediated killing of MT1/2 skin papilloma cells when p50 homodimer activity is elevated. p105(sr) is an effective NF-&#x3ba;B super repressor with a broader range than other currently available IkB&#x3b1; super repressors. The novel repressor can be used in cells where a noncanical NF-&#x3ba;B activity is dominant or multiple NF-&#x3ba;B activities are activated.

Methods and compositions for inhibition of nuclear factor kappaB

Described herein are nucleic acid modules for cloning, expression and tagging of eukaryotic membrane proteins. The nucleic acid modules include a receptor for advanced glycation end products (RAGE) signal sequence, a nucleic acid sequence encoding a tag and a multiple cloning sequence (MCS). Any membrane protein of interest can be cloned into the MCS for expression in cells. The nucleic acid modules can encode any type of tag, such as an epitope tag or affinity tag. The nucleic acid modules disclosed herein can be used to express any type of membrane protein and are particularly suited to the expression and tagging of Type I and Type III membrane proteins.

Nucleic acid modules for expression and tagging of membrane proteins and methods of use

Growing community of inventors

Baltimore, MD, United States of America

Li Lin