Monsanto Technology LLC

Lex Evan Flagel

Michael John Crawford

Christina Marie Taylor

Mahak Kapoor

Brian Donovan Eads

Jodi Lynn Beattie

James Arthur Baum

James Kevin Roberts

David V Butruille

Thomas Clark

Chitvan Khajuria

Gerrit Cornelis Segers

Brian C McNulty

William Moar

Cara Vazquez

Mao Chen

Peter D Jensen

Exemplary systems and methods for selecting from population of candidate edits and predicting an aggregate effect of the candidate edits are disclosed. One exemplary method includes identifying a population of candidate edits to a genomic sequence of said organism and ranking each of the candidate edits based on a predicted ability of each candidate edit to affect a trait of interest in said organism. The exemplary method further includes selecting one or more of the candidate edits based on the ranking and predicting, by the computing device, an aggregate effect of the selected one or more of the candidate edits for the trait of interest when expressed by a specimen of the organism having a genomic sequence and edited according to the selected one or more of the candidate edits, as compared to an unedited specimen of the organism.

Systems and methods for use in identifying multiple genome edits and predicting the aggregate effects of the identified genome edits

Disclosed herein are methods of controlling insect pests, in particularspp. which infest crop plants, and methods of providing plants resistant to such pests. Also disclosed are polynucleotides and recombinant DNA molecules and constructs useful in such methods, insecticidal compositions such as topical sprays containing insecticidal double-stranded RNAs, and solanaceous plants with improved resistance to infestation byspp. Further disclosed are methods of selecting target genes for RNAi-mediated silencing and control ofspp.

Compositions and methods for controlling

The present invention provides methods for controlling invertebrate pest infestations, for instance in plants, and related compositions and polynucleotides useful in such methods. More specifically, the present invention provides polynucleotides and methods of use thereof for modifying the expression of genes in an invertebrate pest, for instance through RNA interference.

The present invention is directed to a method for producing an RNAi resistant insect pest species colony from an insect pest species isolated from an agricultural field. The invention is also directed to methods for determining the inheritance of resistance to the RNAi and determining the allele frequency of resistance in an agricultural field.

Method for producing an RNAi resistant colony of an insect pest species from a field-derived population

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Compositions and methods for controlling leptinotarsa

Growing community of inventors

St. Louis, MO, United States of America

Lex Evan Flagel