University of Michigan

The University of Bath

Cayman Chemical Company Incorporated

Levi Lynn Blazer

Stephen Douglas Barrett

Scott D Larsen

Gregory William Endres

Fred Lawrence Ciske

Stephen Husbands

Richard Neubig

Raymond C Trievel

Jeffrey Keith Johnson

Gregory Scott Keyes

Ranjinder Singh Sidhu

Margaret Lynn Collins

John Traynor

Daniel Austin Bochar

Assay methods may generally comprise forming homogeneous assay mixtures comprising target SAM-utilizing protein, fluorescent detection analyte, and test compound, incubating, and measuring FP or TR-FRET signal emitted in order to determine a measure of test compound-SAM -utilizing protein binding. Assay mixtures comprise a SAM-utilizing protein, and a fluorescent detection analyte that binds with the SAM-utilizing protein in the absence of test compound. Assay mixtures may further comprise a test compound. Assay mixture embodiments may generate FP or TR-FRET signal properties that are a function of the inherent binding interactions of both the test compound and the detection analyte with the SAM-utilizing protein. Fluorescent detection analytes comprise a fluorophore moiety, a covalent linker moiety, and a SAM-utilizing protein ligand moiety and could be utilized in FP or TR-FRET assays to measure test compound binding.

Fluorescent molecular probes for use in assays that measure test compound competitive binding with SAM-utilizing proteins

The invention relates to compositions having RGS (regulator of G-protein Signaling) inhibiting activity, and methods of use thereof. In some embodiments, RGS-inhibiting compositions find use in research on or treatment of disease states (e.g., diabetes, epilepsy, neuropathic pain, depression and other diseases).

Small molecule inhibitors of RGS proteins

Growing community of inventors

Ann Arbor, MI, United States of America

Levi Lynn Blazer