Clemson University

Other

Leslie Sierad

Dan T Simionescu

Agneta Simionescu

Richard Pascal, Iii

Eliza Laine Shaw

George Fercana

Christopher Delaney

Samuel G Rankin

Chris deBorde

Christopher deBorde

Bioreactors and components of bioreactors are described as may be beneficially utilized in development and conditioning of cellular materials for study or implant. The bioreactors are modular, and components of the bioreactors can be easily assembled with alternatives provided to develop specific, predetermined conditioning environments for cellular materials (e.g., implantable tissue). By selection of one of multiple alternative compliance chambers, a bioreactor can be utilized to condition tissue in a low-pressure circuit (e.g., a pulmonary heart circuit), and by utilization of an alternative compliance chamber, the bioreactor can instead condition tissue in a high-pressure circuit (e.g., an aortic heart circuit).

Modular bioreactor, compliance chamber for a bioreactor, and cell seeding apparatus

Bioreactors and components of bioreactors are described as may be beneficially utilized in development and conditioning of cellular materials for study or implant. The bioreactors are modular and components of the bioreactors can be easily assembled with alternatives provided to develop specific, predetermined conditioning environments for cellular materials (e.g., implantable tissue). By selection of one of multiple alternative compliance chambers, a bioreactor can be utilized to condition tissue in a low pressure circuit (e.g., a pulmonary heart circuit), and by utilization of an alternative compliance chamber, the bioreactor can instead condition tissue in a high pressure circuit (e.g., an aortic heart circuit).

Systems and methods that establish a pressure differential across a tissue wall to encourage complete decellularization of the wall are described. The methods can be utilized for decellularization of blood vessel tissue including heart valves and surrounding tissues. The methods and systems can essentially completely decellularize the treated tissue segments. Systems can be utilized to decellularize one or multiple tissue segments at a single time.

Decellularization method and system and decellularized tissue formed thereby

Tissue holders that can be used for gripping natural or synthetic heart valves are described. The tissue holder can include a clamping mechanism and a spring and can be self-adjusting with regard to pressure applied to the tissue gripped in the holder. The tissue holder can be removably attached to systems for processing the tissues and can provide completely hands-free processing of a tissue from development or excisement to implantation and/or completion of testing.

Self-adjusting tissue holder

A male fitting has a body that defines a fluid passage that extends through the body from a first end to a second end. The body may include a first portion adjacent to the first end, a second portion adjacent to the second end and a middle portion separating the first and second portions. The body may further define a slot between the middle portion and the second end. The slot extends at least partially circumferentially around the body. An actuating member may extend at least partially around the body. The actuating member may have at least one stop feature that engages with the body, and a retaining feature that is at least partially aligned with the slot. A spring disposed between the body and the actuating member may exert a force on the actuating member so as to position the retaining feature radially outward from the slot.

Growing community of inventors

Central, SC, United States of America

Leslie Sierad