University of Connecticut

The State University of New York

Leslie M Loew

Joseph P Wuskell

Arvydas Matiukas

Arkady M Pertsov

James C Schaff

John Carson

Ping Yan

Mark A Sapia

Frank R Morgan

Ian R Greenshields

Corey D Acker

The present invention provides a method of optical electrophysiological probing, including: providing a fluorescing chemical probe; contacting a thick portion of tissue with the fluorescing chemical probe to create a thick portion of treated tissue; applying a first range of wavelengths of electromagnetic radiation to the treated portion of tissue; and detecting a plurality of depth-specific emission wavelengths emitted from the thick portion of treated tissue.

Composition, method, system and kit for optical electrophysiology

A fluorinated voltage sensitive dye has the structure wherein p is 0, 1, or 2; Xis an anionic counterion having a charge, q, that is 1 or 2; n is 1 or 2; Ris an optionally substituted C-C, alkyl; Ris hydrogen, and Ris hydrogen or fluorine; or Rand Rcollectively form a divalent &#x2014;CH&#x2550;CH&#x2014;CH&#x2550;CH&#x2014; group; Rand each occurrence of Rare each independently hydrogen or fluorine; Ris hydrogen or fluorine or trifluoromethyl; and each occurrence of Ris independently C-Calkyl;provided that the dye comprises at least one fluorine atom. The dye is particularly useful for monitoring the dynamics of action potentials in axons and/or dendrites.

Fluorinated voltage sensitive dyes, preparation thereof, and optical methods of use

Composition, method, system, and kit for optical electrophysiology

Amino(oligo)thiophene dyes useful for studying the electrophysiology of organelles, cells, and tissues are described. Compared to previously known dyes, the amino(oligo)thiophene dyes exhibit improved (faster) response to membrane potential changes, as well as the ability to be excited by 1064 nanometer femtosecond pulses. Methods of preparing the amino( oligo )thiophene dyes are also described.

Amino(oligo)thiophene dyes, preparation thereof, and optical methods of use

A method and apparatus for modeling cellular structure is disclosed. The system incorporates a theoretical hypotheses of cellular physiology into a simulation framework that allows direct comparison of simulation results with experimental data. The description of the biological model is kept independent of the solution by the use of an integrated anatomical and physiological modeling language. This framework allows complex heterogeneous intracellular chemical simulations to be built with little or no knowledge of the underlying numerical methods. Because the simulation results are easily compared to the experimental results, the user can more easily confirm the hypothesis.

Method for modeling cellular structure and function

A method of simulating a biological material.

Method and apparatus for modeling cellular structure and function

An adaptive structure of a Wiener filter is used to deconvolve three-dimensional wide-field microscope images for the purposes of improving spatial resolution and removing out-of-focus light. The filter is a three-dimensional kernel representing a finite-impulse-response (FIR) structure requiring on the order of one thousand (1000) taps or more to achieve an acceptable mean-square-error. Converging to a solution is done in the spatial-domain and therefore does not experience many of the problems of frequency-domain solutions. Alternatively, a three-dimensional kernel representing an infinite-impulse-response (IIR) structure may be employed. An IIR structure typically requires fewer taps to achieve the same or better performance, resulting in higher resolution images with less noise and faster computations.

Growing community of inventors

West Hartford, CT, United States of America

Leslie M Loew