National Institute of Agrobiological Sciences

Leqing Qu

Fumio Takaiwa

An objective of the present invention is to provide promoters having seed-specific promoter activity, and methods of expressing foreign proteins in seeds. The present inventors isolated the promoter of ADP-Glucose pyrophosphorylase which is expressed in rice seeds, constructed binary vectors in which the ADP-Glucose pyrophosphorylase promoter is inserted upstream of the GUS reporter gene, and transformed rice using themethod. GUS expression level was used as an index to examine the site of expression, the expression pattern during seed maturation, and the level of expression in seeds for the ADP-Glucose Pyrophosphorylase promoter. Expression was found in the embryo during early stage of maturation and in the entire seed during maturation. Expression in late stage of maturation in embryo was very high.

AGPase promoter from rice and uses thereof

An objective of the present invention is to provide promoters having seed-specific promoter activity, and methods of expressing foreign proteins in seeds. In particular, a seed specific promoter from the rice glutelin GluB-4 gene is disclosed. The present inventors isolated the promoters of a number of genes that are expressed in rice seeds, constructed binary vectors in which each promoter is inserted upstream of the GUS reporter gene, and transformed rice using themethod. The inventors then used GUS expression level as an index to examine the site of expression, the expression pattern during seed maturation, and the level of expression in seeds for each promoter. They thus discovered promoters with activity specific to a particular site in seeds, and with higher activity than constitutive promoters and known seed-specific promoters.

Seed-specific promoter from the rice glutelin GluB-4 gene and uses thereof

An objective of the present invention is to provide a promoter from the rice 10 kDa Prolaminin gene having seed-specific promoter activity, and methods of expressing foreign proteins in seeds. The present inventors isolated the promoters of a number of genes that are expressed in rice seeds, constructed binary vectors in which each promoter is inserted upstream of the GUS reporter gene, and transformed rice using themethod. The inventors then used GUS expression level as an index to examine the site of expression, the expression pattern during seed maturation, and the level of expression in seeds for each promoter. They thus discovered promoters with activity specific to a particular site in seeds, and with higher activity than constitutive promoters and known seed-specific promoters.

Seed-specific gene promoter from the rice 10 KDa prolaminin gene and uses thereof

An objective of the present invention is to provide promoters having seed-specific promoter activity, and methods of expressing foreign proteins in seeds. The present inventors isolated the promoters of a number of genes that are expressed in rice seeds, constructed binary vectors in which each promoter is inserted upstream of the GUS reporter gene, and transformed rice using themethod. In particular a seed-specific promoter from the rice glutelin GluB-1 gene is disclosed. GUS expression level was used as an index to examine the site of expression, the expression pattern during seed maturation, and the level of expression in seeds for each promoter. Promoters with activity specific to a particular site in seeds, and with higher activity than constitutive promoters and known seed-specific promoters were discovered.

Growing community of inventors

Tsukuba, Japan

Leqing Qu