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Consejo Superior De Investigaciones Cientificas

Pharmacia & Upjohn Ab

Leonor Kremer Baron

Carlos Martinez Alonso

Yngve Elof Hansson

Jos&eacute; Miguel Rodriguez Frade

Jos&eacute; Mario Mellado Garcia

Jose Mario Garcia

Jose Miguel Frade

Carlos Mart&iacute;nez Alonso

Maria Vela Cuenca

Ana Franco Villanueva

Laura Carramolino Fitera

Jose Alberto Garcia Sanz

Sonia Chamorro Perez

Monoclonal antibody

The present invention relates to a method for determinating cytokine receptor, especially growth hormone receptor (GHR), activation, by the use of an antibody capable of discriminating between an activated and an inactive cytokine receptor conformation, especially GHR or growth hormone binding protein (GHBP) conformation, the method comprising: contacting the antibody and the cytokine receptor to form a complex, contacting a candidate ligand to the complex, measuring the antibody binding to inactive cytokine receptor and thereby discriminating between an activated and an inactive conformation. The invention also relates to an antibody directed to the hinge spanning subdomains I and II of the growth hormone receptor extracellular region and which is capable of discriminating between an activated and an inactive receptor conformation. The invention also relates to the use of the antibody for e.g. characterization of growth hormone receptor interactions and for screening for compounds stabilising die dimerization of GHR.

Method for determining cytokine receptor activation by the use of an antibody

The invention relates to monoclonal antibody capable of specific binding to the molecular weight of 20 kDa variant of human growth hormone. This monoclonal antibody has no substantial binding to hGH of molecular weight 22 kDa. The invention also relates to the use of this monoclonal antibody for measurement of hGH 20K, especially in body fluids. The antibodies can be used for detection and quantification of hGH 20K, especially in serum.

Growing community of inventors

Madrid, Spain

Leonor Kremer Baron