Technische Universiteit Eindhoven

Koninklijke Philips Corporation N.V.

U.S. Philips Corporation

Leonardus Josephus Van Ijzendoorn

Menno Willem José Prins

Emilius Willem Adriaan Visser

Peter Zijlstra

Maarten Merkx

Max Rose-Marie Wilhelmus Scheepers

Menno Willem Jose Prins

Jeroen Hans Nieuwenhuis

Wendy Uyen Dittmer

Peter R Prud'homme Van Reine

Peggy De Kievit

Xander Jozef Antoine Janssen

Emillius Willem Adriaan Visser

Provided herein is a method for biosensing a target substance [] using a collection of particles [] tethered to a surface [] by tether molecules [] and a plurality of capture molecules. A concentration of the target substance is determined from the time sequence of individual association/dissociation rates of the capture molecules. Competitive assay configurations are also described.

Biosensor based on capture molecules with different affinities

A method for sensing an analyte uses tethered particle motion. A functionalized particle has a first state in which the functionalized particle is bound to the surface and a second state in which the functionalized particle is not bound to the surface, where the functionalized particle switches between the first and second states depending on the presence and absence of the analyte, thereby changing motion characteristics of the functionalized particle depending on the presence of the analyte. A spatial coordinate parameter of the functionalized particle is measured by a detector, and a processor determines the presence/concentration of the analyte from changes in the measured spatial coordinate parameter.

Biosensor based on a tethered particle

A method for sensing an analyte uses tethered particle motion. A functionalized particle [] has a first state [] in which the functionalized particle is bound to the surface and a second state [] in which the functionalized particle is not bound to the surface, where the functionalized particle switches between the first and second states depending on the presence and absence of the analyte, thereby changing motion characteristics of the functionalized particle depending on the presence of the analyte. A spatial coordinate parameter of the functionalized particle is measured by a detector [], and a processor [] determines the presence/concentration of the analyte from changes in the measured spatial coordinate parameter.

A method and system for measuring agglutination in a target-induced agglutination assay with one or more magnetic particles is performed in a reaction chamber. After the magnetic particles, which are capable of binding to a target are provided in the assay, an agglutination process is performed resulting in agglutinated particles. Further an alternating current magnetic field (H) is applied to the assay. The method further includes measuring an effect of the Hon the one or more magnetic particles unattached to any surface. The measured effect is indicative of one or more agglutination parameters.

Measuring agglutination parameters

Ceramic translucent material mainly comprising densely sintered polycrystalline yttrium aluminum garnet in which between 20 and 1500 ppm by weight of ZrO.sub.2 is added to the yttrium aluminum garnet. When manufacturing an article of such a ceramic translucent material, a thermal treatment is carried out at a temperature of more than 1400.degree. C. in an oxidizing atmosphere after sintering.

Ceramic translucent material, method of manufacturing such a material

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Eindhoven, Netherlands

Leonardus Josephus Van Ijzendoorn