Honeywell International Inc.

Leon Xu

Yuandong Gu

Zhenhong Sun

Yuan Zheng

Jicang Zhou

Embodiments of the present invention relate to a buffer composition for an integrated nucleic acid amplification and hybridization reaction. The buffer comprise about 50-200 mM of a salt, about 10-30 mM Tris-HCl, about 2-10M Water soluble magnesium salt, about 0.05-1.5% surfactant, about 0.05-0.15 mg/ml stabilizing protein about 50-300 nM of one or more primers, about 20-150 uM of one or more dNTPs, about 5-15% glycerine, about 0.5-1.5% formamide and at least about 5 unit/ml polymerase.

Reaction buffer for microarray

A method for detecting a target nucleic acid of a pathogen in a test sample, the method comprising preparing a target nucleic acid detecting reagent and contacting the target nucleic acid detecting reagent with an oligonucleotide microarray. A kit for detecting a target nucleic acid of a pathogen in a test sample is also described. The kit comprises at least one primer pair and an oligonucleotide microarray comprising at least one probe.

Oligonucleotide microarray for identification of pathogens

Embodiments of the invention relate to an active biochip for nucleic acid analysis. The biochip comprises an inlet for introducing a nucleic acid sample, fluid channels, valves in contact with the fluid channels and pumps in contact with the fluid channels and adapted to generate a carrier gas or move a buffer through a portion of the fluid channels. The biochip also includes one or more hydroxyapatite columns for separating a portion of the nucleic acid sample, buffer reservoirs in contact with the fluid channels and positioned near the pumps, air exits, a waste reservoir and a nucleic acid analysis region.

Active biochip for nucleic acid analysis

A microarray reader () comprises a light source (), beam shaping elements () positioned near the light source (), a moving stage () supporting one or more of the light source () and beam shaping elements (), an optical substrate () supporting an immobilized microarray, a reaction chamber () in contact with the optical substrate () and encapsulating buffer solution, a heating/cooling component () in contact with the reaction chamber (), a synchronization circuit, an optical filter () and an imaging sensor () positioned near the optical filter ().

Microarray reader based on evanescent wave detection and method of reading a microarray

The present invention relates to an apparatus for testing multiple sample compounds for their biological effect comprising a porous block having substantially planar top and bottom surfaces. The top surface comprises a plurality of cell adhesive regions and cell dis-adhesive regions and the bottom surface provides multiple sites to load the sample compounds. These sites are located opposite from the cell adhesive regions on the top surface of the porous block. In certain embodiments, the invention further comprises at least one dissolvable layer which provides multiple sites to load the sample compounds.

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Shanghai, China

Leon Xu