Other

Yeda Research and Development Co. Ltd.

Ramot at Tel-aviv University Ltd.

Leon Goldstein

Amihay Freeman

Mordechai Sokolovsky

Yehuda Levin

Shmaryahu Blumberg

Ephraim Katzir (Katchalski)

The present invention relates to novel isocyano derivatives of linear or cross-linked, natural or synthetic macromolecules, the derivatives being of the general formula P--O--Q--NC, wherein P is the macromolecular backbone, the oxygen atom is part of the hydroxy group, Q is a group --(CH.sub.2).sub.n --wherein n is an integer of at least 3 carbon atoms, and up to about 20 carbon atoms, or Q is an aralkyl group, to a process for preparing such derivatives and to a process of linking to same biologically active proteins and to the products thus obtained.

Isonitrile derivatives of macromolecules containing hydroxyl groups

Enzymes are convalently found to anionic or cationic hydrophilic polymeric carriers formed by essentially complete reaction of pendent anhydride groups of ethylene-maleic anhydride copolymer (EMA) with a diamine selected from the group consisting of hydrazine, P, P'-diaminodiphenylmethane and 1,6-diaminohexane to produce resins having pendent amine groups. The EMA-hydrazide and EMA- P,P'-diaminodiphenylmethane resins can be rendered cationic by reaction with N,N-dimethyl-1,3-propanediamine.

Immobilized enzymes

Novel substituted polyamides of the general formula ##EQU1## wherein A and B, which may be identical or different, are the hydrocarbon backbone groups of the formula --(CH.sub.2).sub.n --, where n is an integer, R.sub.2 and R.sub.3 are the residues of an aldehyde R.sub.2 --CHO or of an isocyanide R.sub.3 --N.tbd.C, where R.sub.2 and R.sub.3 are aliphatic, aromatic or heterocyclic residues of the respective aldehyde or isocyanide, or where R.sub.3 is a chromophoric moiety. R.sub.2 and R.sub.3 can be substituted, and amongst the possible substituents there may be R.sub.3 designating --(CH.sub.2).sub.n --NHCO--CHR.sub.2 --N(CO)(R.sub.4)--X, where R.sub.4 is the residue of a monocarboxylic or dicarboxylic acid and X is the lysyl residue of a protein, which may be biologically active, or the residue of a low-molecular weight compound containing a primary amine moiety. Preferred polyamides are various types of nylons. Amongst proteins chemically bound to the polyamide there may be mentioned enzymes, antibodies and peptide hormones. When R.sub.3 is a chromophoric moiety, the product is a chemically dyed polyamide. The invention relates also to a process for the production of such novel substituted polyamides.

Novel substituted polyamides and process for producing them

Growing community of inventors

Rehovot, Israel

Leon Goldstein