University of Michigan

Leng-Chun Chen

Mary-Ann Mycek

William Lloyd

Stephen E Feinberg

Cynthia Marcelo

Diane M Simeone

James M Scheiman

Barbara McKenna

Malavika Chandra

Robert H Wilson

Jeremy Taylor

Oliver Lee

Hyungjin Myra Kim

Shiuhyang Kuo

This disclosure provides a method of determining the viability of at least one cell via quantification of nicotinamide adenine dinucleotide (phosphate) (NAD(P)H), flavin adenine dinucleotide (FAD), and/or collagen. The method includes the steps of contacting the at least one cell with light having a wavelength of from 700 to 900 nm, using two photon excitation, or from 335 to 400 nm, using one photon excitation, to induce an optical response from the NAD(P)H, FAD, and/or collagen and measuring the optical response. The method also includes the step of quantifying one or more of an amount, spatial localization, and/or time-dependent response of the NAD(P)H, FAD, and/or collagen utilizing the optical response.

Method of determining the viability of at least one cell

Multimodal optical spectroscopy systems and methods produce a spectroscopic event to obtain spectroscopic response data from biological tissue, either ex vivo or in vivo, and compare the response data with a model configured to correlate the measured response data and the most probable attributes of the tissue, thus facilitating classification of the tissue based on those attributes.

Multimodal spectroscopic systems and methods for classifying biological tissue

Growing community of inventors

Ann Arbor, MI, United States of America

Leng-Chun Chen