Novo Industri A/s

Lena Elisabeth Zittan

Keith Gibson

Poul Borge Poulsen

Tage Kjaer Nielsen

William Carasik

Iron activation of cell mass of glucose isomerase preparations carried out by direct incorporation of 0.05-2.0% w/w dry basis of iron as a solid water soluble non-toxic iron salt. Preferred compositions are dry state particulate glucose isomerases containing 0.05-2.0% iron, 0.5-3.0% magnesium oxide, and 2-15% dextrose monohydrate all w/w.

Iron containing cell mass glucose isomerase preparation

Continuous enzymatic isomerization of a glucose syrup with a glucose concentration of 30-55% by weight containing less than about 10.sup.-.sup.3 M calcium, less than about 10.sup.-.sup.2 M of Mg.sup.+.sup.+, the Mg.sup.+.sup.+ being in a concentration, whereby the molar ratio of magnesium to calcium is greater than 5, the isomerization taking place at a pH 7.8-8.6 with a total contact time between enzyme and syrup less than about 3.5 hours, preferably less than 2 hours. A convenient temperature range for isomerization is 60.degree.-85.degree. C., preferably 60.degree.-70.degree. C. The syrup has no colbalt added thereto. A preferred practice involves a syrup with very little added magnesium. Post isomerization ion exchange treatment can be avoided. The enzyme is a particulate preparation derived from B. coagulans, preferably by glutaraldehyde reaction with homogenized microorganism cells.

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Kokkedal, Denmark

Lena Elisabeth Zittan