Conoco Inc.

E.i. Dupont De Nemours and Company

Continental Oil Company

Len J Gawel

R Leroy Grimsley

Richard C Ebersole

James M Odom

Thomas K Ng

Chi-Sin Chen

Sulfate reducing bacteria are detected by preparing a lysate so as to release an enzyme essential to derive energy by reduction of sulfate such as adenosine 5'-phosphosulfate reductase (APS reductase), containing the lysate with an antibody for the enzyme, and detecting the presence of the reaction product of the enzyme and the antibody. In one aspect, sulfate reducing bacteria are controlled responsive to such assay. In another aspect, a 'test kit' is provided for carrying out the assay.

Sulfate reducing bacteria determination and control

An underwater storage structure comprising an internally segmented tetrahedron and/or hexahedron, said self supporting structure having a fluid impermeable covering and an internally segmented skeleton. The structure can be subdivided internally by fluid impermeable coverings allowing ballast, such as water or sand slurry or the like, to be utilized to counter the bouyancy of internal voids formed by the subdivisions of the structure.

Pyramidal offshore structure

Tethered or bottom-ballasted offshore platforms preferably have a pyramidal configuration. Struts connect the apexes of a polyhedron configuration to form a triangle defining each face. The pyramidal configuration can be ballasted to the bottom in relatively shallow water. A hexadron configuration can be tethered in deep water with tension members and flotation members.

A method of producing lipase and pyocyanine from n-paraffins by growing a pseudomonas-like hydrocarbon oxidizing bacteria thereon and extracting the lipase and pyocyanine from the intracellular portion of the bacterium.

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Ponca City, OK, United States of America

Len J Gawel