Illumina Corporation

Spatial Transcriptomics Ab

Spatial Transcriptomics Ab and Illumina, Inc.

10x Genomics Sweden Ab

Leila Bazargan

Alex Aravanis

Gordon M Cann

Joakim Lundeberg

Jonas Frisen

Patrik Stahl

Frank J Steemers

Jeffrey G Mandell

Dmitry K Pokholok

Steven Norberg

Farnaz Absalan

Patrik Ståhl

Jonas Frisén

Dimitry K Pokholok

A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.

Spatially distinguished, multiplex nucleic acid analysis of biological specimens

A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.

Polynucleotide enrichment using CRISPR-Cas systems

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San Diego, CA, United States of America

Leila Bazargan