R.j. Reynolds Tobacco Company

Leigh Hagan

John-Paul Mua

Barry Bratcher

Joshua D Morton

Kyle Ford

Samuel Mark Debusk

Leigh Ann Blevins Joyce

Margarette Elisa Lovette

The present disclosure describes methods of obtaining and/or deriving proteins from plants of thespecies and methods for incorporation of such proteins into various products. For example, a method for obtaining a protein-enriched material from a plant of thespecies or portion thereof is provided, comprising: extracting one or more proteins from the plant material into a solvent to form a liquid protein-containing extract; separating a solid extracted plant material from the liquid protein-containing extract; clarifying the liquid protein-containing extract to form a clarified protein-containing extract and a solids fraction; and treating the clarified protein-containing extract so as to provide a protein-enriched material comprising at least about 60% protein by weight.

Protein-enriched tobacco-derived composition

The present disclosure describes methods of obtaining and/or deriving a sugar-enriched extract from plants of thespecies and methods for incorporation of such extracts into various tobacco compositions. One exemplary method for preparing a sugar-enriched extract comprises contactingplant material with a solvent and extracting sugars into the solvent to form a liquid sugar-containing extract; separating a solid extracted plant material from the liquid sugar-containing extract; clarifying the liquid sugar-containing extract to form a clarified sugar-containing extract and a solids fraction; and isolating the liquid sugar-containing extract to provide a sugar-enriched extract comprising a combined weight of fructose and glucose of at least about 10% by dry weight. In certain embodiments, the sugars of the extract comprise at least about 50% by weight fructose.

Sugar-enriched extract derived from tobacco

Growing community of inventors

Owensboro, KY, United States of America

Leigh Hagan