Pharmacia Diagnostics Ab

Leif E Wide

Henning R Hallgren

A method of indicating rheumatoid factors, primarily such rheumatoid factors which belong to immunoglobulin classes other than the immunoglobulin class IgM, in an aqueous sample. The sample is reacted in the presence of the complement factor C1q in non-bound form with soluble, aggregated immunoglobulin labelled with one or more analytically indicatable atoms or groups to selectively precipitate such rheumatoid factors which, in the presence of C1q, are able to precipitate aggregated immunoglobulin, whereafter the analytically indicatable atoms or groups are indicated in the precipitation phase and/or in the solution.

Method of indicating rheumatoid factors

A method of indicating rheumatoid factors belonging to at least one of the immunoglobulin classes IgM, IgG and IgA in an aqueous sample is disclosed. According to this method any complement factor C1q present in the sample is pacified in a manner known per se, whereafter the sample is reacted with soluble, aggregated immunoglobulin labelled with one or more analytically indictable atoms or groups to form aggregates between rheumatoid factors and the aggregated, labelled immunoglobulin, said aggregate being precipitated out, whereafter the precipitate is separated and the analytically indicatable atoms or groups are indicated in the precipitation phase and/or in the solution.

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Uppsala, Sweden

Leif E Wide