Enzymaster (Ningbo) Bio-Engineering Co., Ltd.

Lei Sun

Haibin Chen

Yong Koy Bong

Baoqin Cai

Chengxiao Zhang

Shumin Shi

Marco Bocola

Chuanyang Shang

Yingxin Zhang

Zhaoqi Zhang

Zikun Wang

Kuifang He

Qinli Peng

Wenhui Zheng

Ruimei Hong

Provided are efficient catalyst of engineered enzymes and an economical enzymatic reaction solution to solve the problems in the current production process of L-tyrosine and its derivatives. The method of the invention has the advantages of high product concentration, mild reaction conditions, simple purification process, simple operation, environmental friendliness, and easy industrial scale-up. Thus, it has good industrial application prospects.

Engineered polypeptides and its application in the synthesis of tyrosine or tyrosine derivatives

Provided are amino acid sequences of ketoreductase polypeptides that are useful for asymmetrically synthesizing chiral alcohol compounds, its preparation process as well as reaction process under industrial-relevant conditions. Also provided are polynucleotide sequences encoding engineered ketoreductase polypeptides, engineered host cells capable of expressing engineered ketoreductase polypeptides, and methods of producing chiral alcohol compounds using engineered ketoreductase polypeptides. Compared to other enzymes, the engineered ketoreductase polypeptides provided by the invention have better catalytic activity and thermal stability, allowing purification of the enzyme solution by heat treatment, which is advantageous for the production of enzymes and the industrial application of enzymatic reactions. The use of the engineered polypeptides of the present invention greatly simplifies the production process of chiral alcohol compounds, reduces the cost of production and the impact on the environment, and has good industrial application prospects.

Engineered ketoreductase polypeptides and uses thereof

The present invention provides amino acid sequences of engineered decarboxylase polypeptides that are useful for catalyzing the decarboxylation of L-aspartate to produce &#x3b2;-alanine, and the preparation process of engineered decarboxylase polypeptides as well as reaction process under industrial-relevant conditions. The present disclosure also provides polynucleotide sequences encoding engineered decarboxylase polypeptides, engineered host cells capable of expressing engineered decarboxylase polypeptides, and methods of producing &#x3b2;-alanine using the engineered cells. Compared to the wild-type decarboxylase, the engineered decarboxylase polypeptide provided by the invention has better activity and stability, and overcomes the inhibition by L-aspartic acid and/or &#x3b2;-alanine. The use of the engineered polypeptides of the present invention for the preparation of &#x3b2;-alanine results in higher unit activity, lower cost, and has good industrial application prospects.

Engineered decarboxylase polypeptides and their application in preparing beta-alanine

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Ningbo, China

Lei Sun