Qingdao University of Science and Technology

Qingdao Agricultural University

Qingdao Doublestar Tire Industrial Co., Ltd. (cn)

Lei Han

Zhen Wang

Jun Wang

Xin Li

Jianbo Jia

Jianguang Qi

Limei Zhong

Zhaoyou Zhu

Yinglong Wang

Peizhe Cui

Fanqing Meng

Kaiguang Wang

Zhonghui Zheng

Lihua Ma

Wenhui Xu

Xiaohong Li

Ying Wei

Guiqin Sun

Xiuzhong Wang

Yucui Zhang

Feng Zhang

Shuli Yin

Hongxia Wang

Provided is a method for continuous detection of a miRNA by a metal-organic framework (MOF)-based nanozyme, including the following steps: step 1, conducting a duplex-specific nuclease (DSN)-assisted signal amplification strategy; and step 2, conducting the flow analysis on a target using a flow injection-batch method, and recording a differential pulse voltammetry (DPV) response of a biosensor in a batch mode; and step 3, in the flow mode, conducting competitive elution on a MOF/indium tin oxide (ITO) electrode with a complementary DNA (cDNA) as a mobile phase. In the present invention, a two-dimensional MOF-based nanozyme is simply synthesized by an ultrasonic method and then applied to a homogeneous electrochemical biosensor without a complicated electrode modification process, showing a continuous detection function, a high selectivity and a desirable sensitivity.

Homogeneous detection method for continuous detection of miRNA by metal-organic framework (MOF)-based nanozyme

A process and a device for continuous flow side-chain alkylation which relate to the technical field of organic synthesis. In this process and the device for continuous flow side-chain alkylation, an ibuprofen raw material is prepared with alkylbenzene as a raw material. This raw material alkylbenzene is easily available and has a low cost, and is suitable for scale-up production. Moreover, an entire preparation process adopts continuous chemical synthesis, and a reaction time of each stage can be precisely controlled, which is beneficial to control a total reaction time and reduce an amount of impurities produced. In this way, a purity and a yield of the ibuprofen raw material are improved. In summary, a continuous synthesis method for side-chain alkylation of alkylbenzene provided by the present disclosure shows a low cost and a high yield.

Process and device for continuous flow side-chain alkylation

Trailer tire

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Qingdao, China

Lei Han