Gist-brocades B.v.

Danisco Ingredients A/s (danisco A/s)

Dsm N.v.

Gist-brocades N.v.

Dupont Nutrition Biosciences Aps

Dsm IP Assets B.v.

Leendert Hendrik De Graaff

Jacob B Visser

Henriette C Van Den Broeck

Francois Strozyk

Johannes C Boonman

Felix J Kormelink

Albert J Van Ooijen

Albert J J Van Ooijen

Marcus Matheus Gielkens

Monique Josina Van Der Wouw

Henri&euml;tte C Van Den Broeck

Henri&euml;tta Catharina Van Den Broeck

Abraham Harder

Peter Michael Andreoli

Janna Gardina Bakhuis

Margaretha Van Heuvel

Yves Coutel

Peter Van Der Veen

Michel Johannes Flipphi

Henriëtta Catharina Van Den Broeck

Henrietta Catharina Van Den Broeck

No&euml;l Nicolaas Maria Elisabeth Van Peij

Marcus Matheus Catharina Gielkens

Henriette Catharina Van Den Broeck

Albert Johannes Joseph Van Ooyen

Jan Dirk René Hille

The subject invention lies in the field of microorganism mutation and selection of the mutants. In particular, the invention is directed at obtaining metabolic mutants in a simple, direct and specific manner. In a preferred embodiment it is also possible to obtain desired mutants not comprising recombinant DNA, thereby facilitating incorporation thereof in products for human consumption or application, due to shorter legislative procedures. The method according to the invention involves random mutation and specific selection of the desired metabolic mutant. Knockout, mutants wherein a gene associated with metabolism is absent or inactivated and mutants with increased or decreased DNA binding capacity are also claimed.

Method to isolate mutants and to clone the complementing gene

The subject invention lies in the field of microorganism mutation and selection of the mutants. In particular, the invention is directed at obtaining metabolic mutants in a simple, direct and specific manner. In a preferred embodiment it is also possible to obtain desired mutants not comprising recombinant DNA, thereby facilitating incorporation thereof in products for human consumption or application, due to shorter legislative procedures. The method according to the invention involves random mutation and specific selection of the desired metabolic mutant. Knockout mutants wherein a gene associated with metabolism is absent or inactivated and mutants with increased or decreased DNA binding capacity are also claimed.

Two cellobiohydrolase polypeptides (CBHA and CBHB) derived from Aspergillus are described and can be used to degrade cellulose. Variants of these peptides are described as well as DNA encoding the peptides, vectors and host cells. The peptides can be used to produce or process food, animal feed, wood pulp, paper and textiles.

Cellulose degrading enzymes of aspergillus

The present invention provides polypeptides with cellulase activity, corresponding DNA sequences, vectors, transformed hosts and a method for the production of these polypeptides, which are obtainable from,or

Fungal cellulases

A nucleotide sequence is provided which encodes a peptide having &bgr;-xylosidase activity and exhibits at least 30% amino acid identity with the amino acid sequence shown in SEQ ID NO. 1 and described in SEQ ID NO. 3 or hybridises under stringent conditions with a nucleotide sequence shown in SEQ ID NO. 1, or a part thereof having at least 15 nucleotides encoding an amino acid sequence shown in SEQ ID NO. 1 and described in SEQ ID NO. 3. Also provided is a peptide having &bgr;-xylosidase activity and exhibiting at least 30% amino acid identity with the amino acid sequence shown in SEQ ID NO. 1 and described in SEQ ID NO. 3 or a part thereof having at least 8 amino acids shown in SEQ ID NO. 1 and described in SEQ ID NO. 3.

&bgr;-xylosidase, nucleotide sequence encoding it, and use thereof

The present invention relates to peptides with multiple enzymatic activitives: carboxymethyl cellusase(CMCase), endoglucanase and &bgr;-glucanase, corresponding nucleotides, vectors and transformed hosts, methods of preparation and use. The peptide is used in the food industry for the liquefaction of plant cell material, e.g., in vegetable or fruit juice manufacture, or as processing aids to reduce the fouling of membranes.

The subject invention lies in the field of microorganism mutation and selection of the mutants. In particular, the invention is directed at obtaining metabolic mutants in a simple, direct and specific manner. In a preferred embodiment it is also possible to obtain desired mutants not comprising recombinant DNA, thereby facilitating incorporation thereof in products for human consumption or application, due to shorter legislative procedures. The method according to the invention involves random mutation and specific selection of the desired metabolic mutant. A nucleic acid cassette comprising a nucleic acid sequence encoding a bidirectional marker, said nucleic acid cassette further comprising a basic transcriptional unit operatively linked to the nucleic acid sequence encoding the bidirectional marker and said nucleic acid cassette further comprising an inducible enhancer or activator sequence linked to the basic transcription unit in such a manner that upon induction of the enhancer or activator sequence the bidirectional marker encoding nucleic acid sequence is expressed, said inducible enhancer or activator sequence being driven from a gene associated with metabolism is claimed as is application thereof in a selection method for mutants. In addition a regular gene xlnR encoding an activating regulator of an inducible enhancer or activator sequence and application of said gene and/or its expression product in overexpression of homologous or heterologous protein or peptide is described. Knockout mutants wherein said gene is absent or inactivated and mutants with increased or decreased DNA binding capacity are also claimed.

An arabinoxylan-degrading enzyme from Aspergillus is provided. The enzyme is active in the degradation of water-insoluble solids obtained from maize. The enzyme can be used in the preparation of animal feed compositions, human food or in industrial processes.

Arabinoxylan degrading enzymes

Methods and DNA constructs are provided for the expression of a fungal acetyl xylan esterase gene in microbial hosts. A purified fungal acetyl xylan esterase is obtained which is suited for the use as an accessory enzyme in the degradation of acetylated xylans.

Method to alter the properties of acetylated xylan

The present invention provides the cloning and expression of purified and isolated DNA molecules, obtainable from fungi, which encode enzymes having arabinan-degrading activity. The present invention also provides DNA constructs containing these DNA molecules and methods for their enhanced expression in selected microbial host cells. The present invention further provides methods for the use of the thus-produced arabinan-degrading enzymes.

Cloning and expression of DNA molecules incoding arabinan-degrading

Cloning and expression of acetyl xylan esterases from fungal origin

Growing community of inventors

Oosterbeek, Netherlands

Leendert Hendrik De Graaff