Cornell Research Foundation Inc.

University of California

The United States Government As Represented by the Department of Veterans Affairs

Fundacao Oswaldo Cruz-fiocruz

Other

Lee W Riley

David A Haake

James Matsunaga

Julio Henrique Rosa Croda

Tracy Ann Young

Isadora Cristina Siqueira

Albert I Ko

Mitermayer Galvão Reis

Michele Barocchi

Michele Anne Barocchi

Pele Chong

Richard A Mathies

Carl F Nathan

Niren Murthy

Sangwei Lu

Jungkyu Kim

Tara Renee BeBoer

Mitermayer Galvào Reis

Sabine Ehrt

Amador Goodridge

Robert Eugene Snyder

Tara Renee DeBoer

Diagnostic assays and methods employ a dual-enzyme cascade system comprised of two enzymatic amplifiers and two molecular probes, by (a) incubating a target enzyme with a target enzyme substrate to liberate a thiol; (b) incubating the thiol with a disulfide inactivated amplification enzyme to activate the amplification enzyme in an interchange reaction of the thiol and the disulfide; and (c) incubating the activated amplification enzyme with an amplification enzyme substrate to generate an amplified signal.

Amplification technology using dual enzyme cascade detection

The invention relates to three isolated DNA molecules that encode for proteins, BigL1, BigL2 and BigL3, in thesp bacterium which have repetitive Bacterial-Ig-like (Big) domains and their use in diagnostic, therapeutic and vaccine applications. According to the present invention, the isolated molecules encoding for BigL1, BigL2 and BigL3 proteins are used for the diagnosis and prevention of infection withspecies that are capable of producing disease in humans and other mammals, including those of veterinary importance.

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The present disclosure provides methods of detectingin an individual, generally involving detecting antibody to a mycobacterial lipid in a biological sample obtained from the individual. The present disclosure further provides compositions and kits for carrying out the methods.

Compositions and methods for detecting mycobacterium

Proteins with repetitive bacterial-Ig-like (Big) domains present in leptospira species

One aspect of the present invention relates to a nucleic acid construct which includes a first nucleic acid and a second nucleic acid operatively coupled to the first nucleic acid. The first nucleic acid molecule encodes a first peptide that has the sequence of InvX or another sequence incorporating the 58 amino acid cellular import region of Mce1A and confers onan ability to enter mammalian cells. The second nucleic acid molecule encoding a second peptide. Expression of the nucleic acid construct produces a fusion protein comprising the first peptide coupled to the second peptide. The second peptide may be a therapeutic or a diagnostic peptide. An alternative embodiment of the nucleic acid construct includes the first nucleic acid and an insertion site suitable for incorporation of the second nucleic acid molecule into this version of the construct. The present invention further relates to the fusion protein per se, screening methods for identifying fusion proteins having a protective effect against a pathogen, and therapeutic fusion proteins.

Cellular delivery agent

The present invention relates to a DNA molecule conferring on an ability to enter mammalian cells and to survive within macrophages. The protein encoded by this gene fragment is useful in vaccines to prevent infection by , while the antibodies raised against this protein can be employed in passively immunizing those already infected by the organism. Both these proteins and antibodies may be utilized in diagnostic assays to detect in tissue or bodily fluids. The protein of the present invention can be associated with various other therapeutic materials, for administration to mammals, particularly humans, to achieve uptake of those materials by such cells.

DNA molecule encoding for cellular uptake of Mycobacterium tuberculosis and uses thereof

Growing community of inventors

Berkeley, CA, United States of America

Lee W Riley