The Cleveland Clinic Foundation

The Ohio State University

Other

Lee Robert Moore

Maciej Zborowski

Jeff Chalmers

Jeffrey J Chalmers

A disposable kit for use in directing fluid through a biological cell separator device (). The kit generally includes a separator tube (), a buffer fluid container (), cell sample container (), separated cell container (), and flushing fluid container (), as well as various conduits () for connecting the containers () and separator tube () in fluid communication together. A cell separator system is provided including a separator tube (), magnet (), pump () and a motorized drive unit (). The motorized drive unit () is operatively connected to the magnet () to allow the magnet () to be moved a sufficient distance away from the separator tube () so as to allow cells adhered to the inside surface thereof to be flushed out of the tube ().

Biological cell separator and disposable kit

The present invention provides methods and apparatuses for determining at least one of a plurality of particle physical characteristics. The particle physical characteristics include particle size, shape, magnetic susceptibility, magnetic label density, charge separation, dielectric constant, and derivatives thereof. The method includes generating a region of space having a substantially constant force field, determining the velocity of at least one particle within the region by identifying and locating the particle and its coordinates in at least two temporally defined digital images, and determining the particle physical characteristics from the determined velocity and a predetermined force field magnitude and direction. A device for determining one or more particle physical characteristics is described which has a force field device for subjecting at least one particle to at least one force field, a substantially transparent flow channel, and a computer system for gathering and analyzing data associated with the at least one particle. A system for determining one or more particle physical characteristics is provided which has a force field device for generating at least one force field having a predetermined force field magnitude and direction and for subjecting at least one particle to the at least one force field, a flow system for regulating the introduction of the at least one particle into the force field device, and a computer system for gathering and analyzing data associated with the at least one particle. A pole piece assembly for producing region of space having a substantially constant magnetic force field is also provided.

System and device for determining particle characteristics

The present invention provides apparatuses and methods for continuously separating particles of interest from a heterogeneous particle population. The heterogeneity can be based on, for example, magnetic susceptibility, particle size, thermal diffusion, phase solubility, and combinations of the preceding. Based on the heterogeneity, a separation force capable of exploiting the separand (i.e., particles subject to the separation process) is provided. The various embodiments of the present invention preferably employ an annular separation channel, appropriate separation force and flow compartments. In a first embodiment, an annular separation channel having semi-permeable inner and outer cylindrical walls is used to generate lateral convection forces. In a second embodiment of the present invention, an annular separation channel having heat conductive inner and outer cylindrical walls is used to generate thermal diffusion forces. In a third embodiment, an annular separation channel having non-permeable inner and outer cylindrical walls is used to generate a solubility difference separation force based on solubility coefficient differences between phases of flow compartments. In a fourth embodiment, an annular separation channel having electrically conductive inner and outer cylindrical walls is used to generate an electrophoretic separation force. In a fifth embodiment, an annular separation channel having electrically conductive inner and outer cylindrical walls is used to generate a dielectrophoretic separation force. Yet other embodiments of the present invention provide for the combination of separation forces including lateral convection, thermal diffusion, solubility difference, electrophoretic, dielectrophoretic, and magnetic separation forces.

Continuous particle and molecule separation with an annular flow channel

The present invention provides methods and apparatuses for determining at least one of a plurality of particle physical characteristics. The particle physical characteristics include particle size, shape, magnetic susceptibility, magnetic label density, charge separation, dielectric constant, and derivatives thereof. The method includes generating a region of space having a substantially constant force field, determining the velocity of at least one particle within the region by identifying and locating the particle and its coordinates in at least two temporally defined digital images, and determining the particle physical characteristics from the determined velocity and a predetermined force field magnitude and direction. A device for determining one or more particle physical characteristics is described which has a force field device for subjecting at least one particle to at least one force field, a substantially transparent flow channel, and a computer system for gathering and analyzing data associated with the at least one particle. A system for determining one or more particle physical characteristics is provided which has a force field device for generating at least one force field having a predetermined force field magnitude and direction and for subjecting at least one particle to the at least one force field, a flow system for regulating the introduction of the at least one particle into the force field device, and a computer system for gathering and analyzing data associated with the at least one particle. A pole piece assembly for producing a region of space having a substantially constant magnetic force field is also provided.

Method for determining particle characteristics

A method and apparatus for the fraction sorting of cells is provided. The invention include a dispensing device, aspirating device, flow channel and a magnetic assembly. A first fractional sorting device includes a dipole fractional cell sorter. The dipole fractional cell sorter includes large aspect ratio flow channel and a dipole magnetic assembly. A second fractional sorting device includes a quadrupole fractional cell sorter. The quadrupole fractional cell sorter includes an annular flow channel having inlet and outlet splitters, and a quadrupole magnetic assembly. The quadrupole magnetic assembly includes pole pieces having projecting members with convex surfaces and in is in physical communication with the annular flow chamber. The method of the present invention provides a flow through fractional cell sorting process which is based on the application of a magnetic force to cells having a range of magnetic labeling densities.

Fractional cell sorter

A method for separating cells is presented. The method provides a flow through fractional cell sorting process that is based on the application of a magnetic force to cells having a range of magnetic labeling densities. Magnetically labeled cells are introduced into a flow channel. The cells are continuously fractionated into flow streams based on magnetic susceptibility by subjecting the cells in the flow channel to a magnetic field. The fractionated cells are recovered based on their response to the magnetic field. Methods employing dipole fractional sorters and quadrapole annular sorters are also presented.

Growing community of inventors

Cleveland Heights, OH, United States of America

Lee Robert Moore