The University of Texas System

LeAnne M Campbell

Keerti S Rathore

Ganesan Sunilkumar

Devendra Pandeya

Sreenath R Palle

Madhusudhana R Janga

A method is disclosed for reducing the level of gossypol in cottonseed. The method generally includes selectively inducing RNA gene silencing in the seed of a transgenic cotton plant, to interfere with expression of the &#x3b4;-cadinene synthase gene or the &#x3b4;-cadinene-8-hydroxylase gene in the seed of the cotton plant without substantially affecting expression of that gene in the foliage, floral parts, and roots of the plant. The transgenic cotton plant comprises at least one of a &#x3b4;-cadinene synthase gene trigger sequence and/or a &#x3b4;-cadinene-8-hydroxylase gene trigger sequence operably linked to one or more a seed-specific promoter gene sequences, and the trigger sequence(s) is/are able to induce RNA gene silencing when expressed in cottonseed of the plant. Also disclosed are expression cassettes, vectors, cells, seeds, and plants containing at least one of a &#x3b4;-cadinene synthase gene trigger sequence and/or a &#x3b4;-cadinene-8-hydroxylase gene trigger sequence operably linked to one ore more a seed-specific promoter DNA sequences.

Cotton plant with seed-specific reduction in gossypol

The present disclosure provides cotton plants with reduced gossypol levels in the seed, and in further embodiments provides cotton plants with increased gossypol levels in the leaves. Also provided are methods for reducing gossypol content in seeds of a cotton plant by down-regulation of CGF2 expression, and in certain embodiments CGF1 and/or CGF3 expression, in the plant, and methods for increasing gossypol content in leaves of a cotton plant by tissue-specific overexpression of CGF2, and in certain embodiments CGF1 and/or CGF3, in the plant.

Methods and compositions for modulating gossypol content in cotton plants

The invention provides cotton event TAM66274, and plants, plant cells, seeds, plant parts, and commodity products comprising event TAM66274. The invention also provides polynucleotides specific for event TAM66274 and plants, plant cells, seeds, plant parts, and commodity products comprising polynucleotides specific for event TAM66274. The invention also provides methods related to event TAM66274.

Cotton transgenic event TAM66274

A method is disclosed for reducing the level of gossypol in cottonseed. The method generally includes selectively inducing RNA gene silencing in the seed of a transgenic cotton plant, to interfere with expression of the &#x3b4;-cadinene synthase gene or the &#x3b4;-cadinene-8-hydroxylase gene in the seed of the cotton plant without substantially affecting expression of that gene in the foliage, floral parts, and roots of the plant. The transgenic cotton plant comprises at least one of a &#x3b4;-cadinene synthase gene trigger sequence and/or a &#x3b4;-cadinene-8-hydroxylase gene trigger sequence operably linked to one or more a seed-specific promoter gene sequences, and the trigger sequence(s) is/are able to induce RNA gene silencing when expressed in cottonseed of the plant. Also disclosed are expression cassettes, vectors, cells, seeds, and plants containing at least one of a &#x3b4;-cadinene synthase gene trigger sequence and/or a &#x3b4;-cadinene-8-hydroxylase gene trigger sequence operably linked to one or more a seed-specific promoter DNA sequences.

Growing community of inventors

Cypress, TX, United States of America

LeAnne M Campbell