Centre National De La Recherche Scientifique

Universite Claude Bernard – Lyon1

Institut National Des Sciences Appliquees De Lyon

Ecole Superieure De Chimie Physique Electronique De Lyon

Lab Skin Creations

Léa Pourchet

Christophe Marquette

Amélie Thepot

Morgan Dos Santos

The invention relates to a method for manufacturing a bio-ink by additive deposition, which comprises supplying: a first solution including between 5 and 40 wt. % gelatin; a second solution including between 15 and 35.wt. % alginate; a third solution including between 1 and 15 wt. % fibrinogen, and optionally living cells in suspension; and creating a mixture including: around 35 to 65 vol. % of the first solution; around 15 to 35 vol. % of the second solution; and around 15 to 35 vol. % of the third solution, said proportions being selected so that they add up to 100%. Said bio-ink allows the additive deposition of objects that can be polymerised by means of a solution including calcium ions and thrombin. Said objects can be incubated and can be used as a substitute for body tissue, for example (with added fibroblasts) as skin substitute.

Method for manufacturing body substitutes by additive deposition

Growing community of inventors

Lyons, France

Léa Pourchet