Rafa Laboratories Ltd.

Lea Grundman

Composition for the determining of whether or not a female is pregnant are provided. The composition consists essentially of a neutral and inert particle on which .gamma.-globulin is adsorbed, said .gamma.-globulin being coupled to a bi-functional coupling agent, the two functional groups thereof possessing different reactivities, the other functional group of said coupling agent being coupled to HCG. The body fluid of the female suspected of being pregnant is mixed with the above composition and permitted to stand. Agglutination indicates non-pregnancy whereas failure of agglutination indicates pregnancy.

Composition and method for determination of pregnancy

Compositions for the determination of whether or not a female animal, and particularly a human female, is pregnant are provided which compositions have prolonged shelf life. The compositions comprise chorionic gonadotropin coupled to animal red blood cells by means of glutaraldehyde as coupling agent therebetween as well as anti-chorionic gonadotropin serum and gelatin which has the effect of greatly increasing the shelf life and accuracy over prolonged periods of time of the composition. The body fluid of the female animal suspected of being pregnant is mixed with the above composition and permitted to stand. Agglutination indicates non-pregnancy whereas failure of agglutination indicates pregnancy. The invention further comprises that the specific use of turkey blood or camel blood as the source of the red blood cells, the use of either turkey blood or camel blood greatly reducing the time required for the testing.

The invention relates to a method of determining the concentration of luteinizing hormone (hereinafter referred to as 'LH') in the body fluid of a woman and thereby determining the day when said woman ovulates, as well as to compositions for effecting this determination. The composition comprises human chorionic gonadotropin (hereinafter referred to as 'HCG') coupled to red blood cells by means of glutaraldehyde as coupling agent therebetween, which composition in carrying out the test is mixed with the body fluid of the woman and an anti-HCG serum in an amount adjusted to react with approximately 50 international units (I.U.) of HCG per liter of the body fluid, generally urine, so that agglutination occurs when LH equivalent to less than 50 I.U. of HCG/L is present and no agglutination when at least 50 I.U. HCG/L is present. The day of highest concentration of LG equivalent to HCG indicating the day of ovulation.

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Jerusalem, Israel

Lea Grundman